Anti-Ms LPAM-1 Purified

Anti-Ms LPAM-1 Purified
Regulatory status
RUO
Antigen
LPAM-1
Clone
DATK32
Format
Purified
Reactivity
Mouse
Application
IP, FC (QC tested), IHC(F)
Variant
0.1 mg
11-747-C100
In stock
80.00 USD

0.025 mg
11-747-C025
Out of stock
40.00 USD
Variant
0.1 mg
11-261-C100
In stock
80.00 USD

0.1 mg
11-261-C100
In stock
40.00 USD
Product details
Images
References
Isotype
Rat IgG2a kappa
Specificity
The rat monoclonal antibody DATK32 recognizes an extracellular epitope of integrin alpha 4 (CD49d) and integrin beta7 (Ly69) components of mouse LPAM-1 complex, which is expressed on the majority of peripheral lymphocytes, as well as on subsets of thymocytes and bone marrow cells.
Application
IP, FC (QC tested), IHC(F)
Reactivity
Mouse
Immunogen
TK1 cells
Other names
Integrin alpha 4 beta 7, CD49d / Ly69
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-747_FC_Profil Značení
Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse LPAM-1 (DATK32) purified antibody (concentration in sample 2 μg/ml) DAR APC.
11-747_FC_Histogram
Separation of murine LPAM-1 positive cells (red-filled) from LPAM-1 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse LPAM-1 (DATK32) purified antibody (concentration in sample 2 μg/ml) DAR APC.

General references:

Reese SR, Kudsk KA, Genton L, Ikeda S: l-selectin and alpha4beta7 integrin, but not ICAM-1, regulate lymphocyte distribution in gut-associated lymphoid tissue of mice. Surgery. 2005 Feb;137(2):209-15.
PubMed
Yang Y, Cardarelli PM, Lehnert K, Rowland S, Krissansen GW: LPAM-1 (integrin alpha 4 beta 7)-ligand binding: overlapping binding sites recognizing VCAM-1, MAdCAM-1 and CS-1 are blocked by fibrinogen, a fibronectin-like polymer and RGD-like cyclic peptides. Eur J Immunol. 1998 Mar;28(3):995-1004.
PubMed
Altevogt P, Hubbe M, Ruppert M, Lohr J, von Hoegen P, Sammar M, Andrew DP, McEvoy L, Humphries MJ, Butcher EC: The alpha 4 integrin chain is a ligand for alpha 4 beta 7 and alpha 4 beta 1. J Exp Med. 1995 Aug 1;182(2):345-55.
PubMed

Product specific references:

Priestley GV, Scott LM, Ulyanova T, Papayannopoulou T: Lack of alpha4 integrin expression in stem cells restricts competitive function and self-renewal activity. Blood. 2006 Apr 1;107(7):2959-67.
PubMed
Riedel SS, Mottok A, Brede C, Bäuerlein CA, Jordán Garrote AL, Ritz M, Mattenheimer K, Rosenwald A, Einsele H, Bogen B, Beilhack A: Non-invasive imaging provides spatiotemporal information on disease progression and response to therapy in a murine model of multiple myeloma. PLoS One. 2012;7(12):e52398.
PubMed
Variant
0.1 mg
11-747-C100
In stock
80.00 USD

0.025 mg
11-747-C025
Out of stock
40.00 USD
Variant
0.1 mg
11-261-C100
In stock
80.00 USD

0.1 mg
11-261-C100
In stock
40.00 USD