Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD90.2 Purified Azide Free

Anti-Ms CD90.2 Purified Azide Free

Regulatory status

RUO

Antigen

CD90.2

Clone

1aG4/C5

Format

Purified Azide Free

Reactivity

Mouse

Application

WB, FC (QC tested), ICC, FUNC

Variant

0.1 mg

10-443-C100

In stock

147.40 USD

0.025 mg

10-443-C025

Delivery 1 week

73.70 USD

Variant

0.1 mg

10-443-C100

In stock

147.40 USD

0.025 mg

10-443-C025

Delivery 1 week

73.70 USD

Contact distributor

Product details

Description

References

SDS download

Isotype

Mouse IgG3

Specificity

The mouse monoclonal antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI anchored glycoprotein (immunoglobulin superfamily); in mouse it is abundant on thymocytes and peripheral T lymphocytes.

Application

WB, FC (QC tested), ICC, FUNC

Application details

Functional application: The bound of antibody 1aG4/C5 to the transfected mast cells induces the activation processes which are similar, but not identical, to the antigen activation of IgE stimulated cells.
Immunoprecipitation: The antibody 1aG4/C5 precipitates complexes with Lyn protein tyrosine kinase.
Flow cytometry: Recommended dilution: 1 μg/ml.

Reactivity

Mouse

Negative species

Rat, Human

Immunogen

thymocytes from C3H/Di mice (Thy-1.2 positive)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Thy-1.2

Antigen description

CD90 (Thy-1) is an 18-35 kDa GPI-anchored plasma membrane glycoprotein expressed in many cell types, such as in hematopoietic cells and neurons, connective tissues, various fibroblast and stromal cell lines, tumor endothelial cell lines and other. In the mouse, CD90 is expressed mainly on thymocytes and peripheral T lymphocytes. It is involved in T cell activation, cellular adhesion, proliferation and migration, neurite outgrowth, wound healing, apoptosis, and fibrosis. CD90 participates in multiple signaling cascades and its effects are tissue- and cell type-specific. It often functions as an important regulator of cell-cell and cell-matrix interactions.

Entrez Gene ID 21838

UniProt ID P01831

General references:

Rege TA, Hagood JS: Thy-1, a versatile modulator of signaling affecting cellular adhesion, proliferation, survival, and cytokine/growth factor responses. Biochim Biophys Acta. 2006 Oct;1763(10):991-9.
PubMed

Haeryfar SM, Hoskin DW: Thy-1: more than a mouse pan-T cell marker. J Immunol. 2004 Sep 15;173(6):3581-8.
PubMed

Rege TA, Hagood JS: Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 2006 Jun;20(8):1045-54.
PubMed

Product specific references:

Draberova L, Draber P: Thy-1 glycoprotein and src-like protein-tyrosine kinase p53/p56lyn are associated in large detergent-resistant complexes in rat basophilic leukemia cells. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3611-5.
PubMed

Bradley JE, Ramirez G, Hagood JS: Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors. 2009 May-Jun;35(3):258-65.
PubMed

Heneberg P, Lebduška P, Draberova L, Korb J, Draber P: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806.
PubMed

Cikán J, Dráber P, Vojtíšková M: Monoclonal IgG3 (kappa) antibodies against murine Thy-1.2 antigen produced by murine hybridomas. Differences in the specificity of the antigen binding site and in the structure of the hinge region. Folia Biol (Praha). 1982;28(6):377-94.
PubMed

Dráber P, Cikán J, Vojtíšková M: Establishment and characterization of permanent murine hybridomas secreting monoclonal anti-thy-1 antibodies. J Immunogenet. 1980 Dec;7(6):455-74.
PubMed