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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Ms CD90.2 PE
Anti-Ms CD90.2 PE
Regulatory status
RUO
Antigen
CD90.2
Clone
1aG4/C5
Format
PE
Reactivity
Mouse
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-443-C100
In stock
100.00 USD
0.025 mg
1P-443-C025
Out of stock
50.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD
0.1 mg
11-261-C100
In stock
50.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG3
Specificity
The mouse monoclonal antibody 1aG4/C5 recognizes alloantigen Thy-1.2 of mouse Thy-1 (CD90); it does not cross-react with Thy-1.1 alloantigen. Thy-1 is an 18-35 kDa cell surface GPI anchored glycoprotein (immunoglobulin superfamily); in mouse it is abundant on thymocytes and peripheral T lymphocytes.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-2 μg/ml.
Reactivity
Mouse
Negative species
Rat, Human
Immunogen
thymocytes from C3H/Di mice (Thy-1.2 positive)
Other names
Thy-1.2
Concentration
0.1 mg/ml
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Flow cytometry analysis (surface staining) of murine splenocytes using anti-CD90.2 (1aG4/C5) PE.
General references:
Rege TA, Hagood JS: Thy-1, a versatile modulator of signaling affecting cellular adhesion, proliferation, survival, and cytokine/growth factor responses. Biochim Biophys Acta. 2006 Oct;1763(10):991-9.
PubMed
Haeryfar SM, Hoskin DW: Thy-1: more than a mouse pan-T cell marker. J Immunol. 2004 Sep 15;173(6):3581-8.
PubMed
Rege TA, Hagood JS: Thy-1 as a regulator of cell-cell and cell-matrix interactions in axon regeneration, apoptosis, adhesion, migration, cancer, and fibrosis. FASEB J. 2006 Jun;20(8):1045-54.
PubMed
Product specific references:
Draberova L, Draber P: Thy-1 glycoprotein and src-like protein-tyrosine kinase p53/p56lyn are associated in large detergent-resistant complexes in rat basophilic leukemia cells. Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3611-5.
PubMed
Bradley JE, Ramirez G, Hagood JS: Roles and regulation of Thy-1, a context-dependent modulator of cell phenotype. Biofactors. 2009 May-Jun;35(3):258-65.
PubMed
Heneberg P, Lebduška P, Draberova L, Korb J, Draber P: Topography of plasma membrane microdomains and its consequences for mast cell signaling. Eur J Immunol. 2006 Oct;36(10):2795-806.
PubMed
Cikán J, Dráber P, Vojtíšková M: Monoclonal IgG3 (kappa) antibodies against murine Thy-1.2 antigen produced by murine hybridomas. Differences in the specificity of the antigen binding site and in the structure of the hinge region. Folia Biol (Praha). 1982;28(6):377-94.
PubMed
Dráber P, Cikán J, Vojtíšková M: Establishment and characterization of permanent murine hybridomas secreting monoclonal anti-thy-1 antibodies. J Immunogenet. 1980 Dec;7(6):455-74.
PubMed
Variant
0.1 mg
1P-443-C100
In stock
100.00 USD
0.025 mg
1P-443-C025
Out of stock
50.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD
0.1 mg
11-261-C100
In stock
50.00 USD
Contact distributor
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