Anti-Ms CD9 PE

Anti-Ms CD9 PE
Regulatory status
RUO
Antigen
CD9
Clone
EM-04
Format
PE
Reactivity
Mouse
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-567-C100
In stock
154.00 USD

0.025 mg
1P-567-C025
Delivery 1 week
66.00 USD
Variant
0.1 mg
1P-567-C100
In stock
154.00 USD

0.025 mg
1P-567-C025
Delivery 1 week
66.00 USD
Product details
Description
Images
References
SDS download
Isotype
Rat IgG1
Specificity
The rat monoclonal antibody EM-04 recognizes an extracellular epitope of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.
Application
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Mouse
Immunogen
Permeabilized murine bone marrow-derived mast cells (BMMC).
Concentration
0.5 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Mic3, Mrp-1, Btcc-1, Drap-27, Tspan-29
Antigen description
CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.
Entrez Gene ID 12527
UniProt ID P40240
1P-567 FC
Flow cytometry analysis (surface staining) of CD9 in murine peritoneal fluid cells with anti-CD9 (EM-04) PE.

General references:

Kotha J, Zhang C, Longhurst CM, Lu Y, Jacobs J, Cheng Y, Jennings LK: Functional relevance of tetraspanin CD9 in vascular smooth muscle cell injury phenotypes: a novel target for the prevention of neointimal hyperplasia. Atherosclerosis. 2009 Apr;203(2):377-86.
PubMed
Liu WM, Cao YJ, Yang YJ, Li J, Hu Z, Duan EK: Tetraspanin CD9 regulates invasion during mouse embryo implantation. J Mol Endocrinol. 2006 Feb;36(1):121-30.
PubMed
Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 1;66(19):9557-65.
PubMed
Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov;98(5):1081-7.
PubMed
Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov;455(2):283-96.
PubMed
Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9.
PubMed

Product specific references:

Athman JJ, Wang Y, McDonald DJ, Boom WH, Harding CV, Wearsch PA: Bacterial membrane vesicles mediate the release of Mycobacterium tuberculosis lipoglycans and lipoproteins from infected macrophages. J Immunol. 2015 Aug 1;195(3):1044-53.
PubMed
Variant
0.1 mg
1P-567-C100
In stock
154.00 USD

0.025 mg
1P-567-C025
Delivery 1 week
66.00 USD
Variant
0.1 mg
1P-567-C100
In stock
154.00 USD

0.025 mg
1P-567-C025
Delivery 1 week
66.00 USD

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