Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD9 Biotin

Anti-Ms CD9 Biotin

Regulatory status

RUO

Antigen

CD9

Clone

EM-04

Format

Biotin

Reactivity

Mouse

Application

IP, WB, FC (QC tested), ICC

Variant

0.1 mg

1B-567-C100

In stock

110.00 USD

Variant

0.1 mg

1B-567-C100

In stock

110.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG1

Specificity

The rat monoclonal antibody EM-04 recognizes an extracellular epitope of CD9 antigen, a 24 kDa transmembrane protein expressed on platelets, monocytes, pre-B lymphocytes, granulocytes and activated T lymphocytes.

Application

IP, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 2-4 μg/ml.

Reactivity

Mouse

Immunogen

Permeabilized murine bone marrow-derived mast cells (BMMC).

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Mic3, Mrp-1, Btcc-1, Drap-27, Tspan-29

Antigen description

CD9 belongs to proteins of tetraspanin family that orchestrate cholesterol-associated tetraspanin-enriched signaling microdomains within the plasma membrane, forming complexes with each other as well as with integrins, membrane-anchored growth factors and other proteins. CD9 is involved in cell motility, osteoclastogenesis, neurite outgrowth, myotube formation, and sperm-egg fusion, plays roles in cell attachment and proliferation and is necessary for association of heterologous MHC II molecules on the dendritic cell plasma membrane which is important for effective T cell stimulation. CD9 is also considered as metastasis suppressor in solid tumors.

Entrez Gene ID 12527

UniProt ID P40240

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC).

Flow cytometry multicolor surface staining pattern of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC) and SYTO16.

Separation of murine myeloid cells (red-filled) from CD9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD9 (EM-04) Biotin antibody (concentration in sample 2 μg/ml, Streptavidin APC).

General references:

Kotha J, Zhang C, Longhurst CM, Lu Y, Jacobs J, Cheng Y, Jennings LK: Functional relevance of tetraspanin CD9 in vascular smooth muscle cell injury phenotypes: a novel target for the prevention of neointimal hyperplasia. Atherosclerosis. 2009 Apr;203(2):377-86.
PubMed

Liu WM, Cao YJ, Yang YJ, Li J, Hu Z, Duan EK: Tetraspanin CD9 regulates invasion during mouse embryo implantation. J Mol Endocrinol. 2006 Feb;36(1):121-30.
PubMed

Saito Y, Tachibana I, Takeda Y, Yamane H, He P, Suzuki M, Minami S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I. Absence of CD9 enhances adhesion-dependent morphologic differentiation, survival, and matrix metalloproteinase-2 production in small cell lung cancer cells. Cancer Res. 2006 Oct 1;66(19):9557-65.
PubMed

Israels SJ, McMillan-Ward EM: Platelet tetraspanin complexes and their association with lipid rafts. Thromb Haemost. 2007 Nov;98(5):1081-7.
PubMed

Kim YJ, Yu JM, Joo HJ, Kim HK, Cho HH, Bae YC, Jung JS: Role of CD9 in proliferation and proangiogenic action of human adipose-derived mesenchymal stem cells. Pflugers Arch. 2007 Nov;455(2):283-96.
PubMed

Unternaehrer JJ, Chow A, Pypaert M, Inaba K, Mellman I: The tetraspanin CD9 mediates lateral association of MHC class II molecules on the dendritic cell surface. Proc Natl Acad Sci U S A. 2007 Jan 2;104(1):234-9.
PubMed

Product specific references:

Athman JJ, Wang Y, McDonald DJ, Boom WH, Harding CV, Wearsch PA: Bacterial membrane vesicles mediate the release of Mycobacterium tuberculosis lipoglycans and lipoproteins from infected macrophages. J Immunol. 2015 Aug 1;195(3):1044-53.
PubMed