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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Ms CD8b PE
Anti-Ms CD8b PE
Regulatory status
RUO
Antigen
CD8b
Clone
H35-17.2
Format
PE
Reactivity
Mouse
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-779-C100
Ask for delivery term
HERE
203.50 USD
0.025 mg
1P-779-C025
Delivery 1 week
101.75 USD
Variant
0.1 mg
1P-779-C100
Ask for delivery term
HERE
203.50 USD
0.025 mg
1P-779-C025
Delivery 1 week
101.75 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Rat IgG2b kappa
Specificity
The rat monoclonal antibody H35-17.2 recognizes an extracellular epitope of mouse CD8b, the 32-34 kDa beta chain of the CD8 coreceptor, expressed on cytotoxic T cells and thymocytes.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Mouse
Immunogen
Mouse CD8b
Concentration
0.5 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Ly-3, Ly-C, Lyt-3
Antigen description
The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
UniProt ID
P10300
Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD8b (H35-17.2) PE antibody (concentration in sample 0.56 μg/ml).
Separation of murine CD8b positive splenocytes (red-filled) from murine CD8b negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8b (H35-17.2) PE antibody (concentration in sample 0.56 μg/ml).
Product specific references:
Cooney LA, Gupta M, Thomas S, Mikolajczak S, Choi KY, Gibson C, Jang IK, Danziger S, Aitchison J, Gardner MJ, Kappe SH, Wang R: Short-lived effector CD8 T cells induced by genetically attenuated malaria parasite vaccination express CD11c. Infect Immun. 2013 Nov;81(11):4171-81
PubMed
Weici Zhang, Masanobu Tsuda, Guo-Xiang Yang, Koichi Tsuneyama, Xiao-Song He, Aftab A. Ansari, William M. Ridgway, Ross L. Coppel, Zhe-Xiong Lian, Patrick S.C. Leung, M. Eric Gershwin: Lymphoma-Like T Cell Infiltration in Liver Is Associated with Increased Copy Number of Dominant Negative Form of TGFβ Receptor II. PLoS One. 2012; 7(11): e49413.
PubMed
Henderson AJ, Kumar A, Barnett B, Dow SW, Ryan EP: Consumption of rice bran increases mucosal immunoglobulin A concentrations and numbers of intestinal Lactobacillus spp. J Med Food. 2012 May;15(5):469-75.
PubMed
Krieg C, Boyman O, Fu YX, Kaye J: B and T lymphocyte attenuator regulates CD8+ T cell-intrinsic homeostasis and memory cell generation. Nat Immunol. 2007 Feb;8(2):162-71.
PubMed
Chaudhary N, Datta K, Askin FB, Staab JF, Marr KA: Cystic fibrosis transmembrane conductance regulator regulates epithelial cell response to Aspergillus and resultant pulmonary inflammation. Am J Respir Crit Care Med. 2012 Feb 1;185(3):301-10.
PubMed
Rubin B, Riond J, Leghait J, Gairin JE: Interactions between CD8alphabeta and the TCRalphabeta/CD3-receptor complex. Scand J Immunol. 2006 Sep;64(3):260-70.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
1P-779-C100
Ask for delivery term
HERE
203.50 USD
0.025 mg
1P-779-C025
Delivery 1 week
101.75 USD
Variant
0.1 mg
1P-779-C100
Ask for delivery term
HERE
203.50 USD
0.025 mg
1P-779-C025
Delivery 1 week
101.75 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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