Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD8b PE

Anti-Ms CD8b PE

Regulatory status

RUO

Antigen

CD8b

Clone

H35-17.2

Format

Reactivity

Mouse

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-779-C100

Ask for delivery term HERE

198.00 USD

0.025 mg

1P-779-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

1P-779-C100

Ask for delivery term HERE

198.00 USD

0.025 mg

1P-779-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2b kappa

Specificity

The rat monoclonal antibody H35-17.2 recognizes an extracellular epitope of mouse CD8b, the 32-34 kDa beta chain of the CD8 coreceptor, expressed on cytotoxic T cells and thymocytes.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Mouse

Immunogen

Mouse CD8b

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Ly-3, Ly-C, Lyt-3

Antigen description

The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

UniProt ID P10300

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD8b (H35-17.2) PE antibody (concentration in sample 0.56 μg/ml).

Separation of murine CD8b positive splenocytes (red-filled) from murine CD8b negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8b (H35-17.2) PE antibody (concentration in sample 0.56 μg/ml).

Product specific references:

Cooney LA, Gupta M, Thomas S, Mikolajczak S, Choi KY, Gibson C, Jang IK, Danziger S, Aitchison J, Gardner MJ, Kappe SH, Wang R: Short-lived effector CD8 T cells induced by genetically attenuated malaria parasite vaccination express CD11c. Infect Immun. 2013 Nov;81(11):4171-81
PubMed

Weici Zhang, Masanobu Tsuda, Guo-Xiang Yang, Koichi Tsuneyama, Xiao-Song He, Aftab A. Ansari, William M. Ridgway, Ross L. Coppel, Zhe-Xiong Lian, Patrick S.C. Leung, M. Eric Gershwin: Lymphoma-Like T Cell Infiltration in Liver Is Associated with Increased Copy Number of Dominant Negative Form of TGFβ Receptor II. PLoS One. 2012; 7(11): e49413.
PubMed

Henderson AJ, Kumar A, Barnett B, Dow SW, Ryan EP: Consumption of rice bran increases mucosal immunoglobulin A concentrations and numbers of intestinal Lactobacillus spp. J Med Food. 2012 May;15(5):469-75.
PubMed

Krieg C, Boyman O, Fu YX, Kaye J: B and T lymphocyte attenuator regulates CD8+ T cell-intrinsic homeostasis and memory cell generation. Nat Immunol. 2007 Feb;8(2):162-71.
PubMed

Chaudhary N, Datta K, Askin FB, Staab JF, Marr KA: Cystic fibrosis transmembrane conductance regulator regulates epithelial cell response to Aspergillus and resultant pulmonary inflammation. Am J Respir Crit Care Med. 2012 Feb 1;185(3):301-10.
PubMed

Rubin B, Riond J, Leghait J, Gairin JE: Interactions between CD8alphabeta and the TCRalphabeta/CD3-receptor complex. Scand J Immunol. 2006 Sep;64(3):260-70.
PubMed