Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD8a Purified

Anti-Ms CD8a Purified

Regulatory status

RUO

Antigen

CD8a

Clone

53-6.7

Format

Purified

Reactivity

Mouse

Application

IP, FC (QC tested), IHC-F, IHC-P, ICC

Variant

0.1 mg

11-579-C100

In stock

88.00 USD

0.025 mg

11-579-C025

Delivery 1 week

44.00 USD

Variant

0.1 mg

11-579-C100

In stock

88.00 USD

0.025 mg

11-579-C025

Delivery 1 week

44.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34 kDa; alpha chain of the CD8 antigen).

Application

IP, FC (QC tested), IHC-F, IHC-P, ICC

Application details

Flow cytometry: Recommended dilution: 1.5 μg/ml.
Immunohistochemistry (frozen sections): Recommended dilution: 1:1000; formaldehyde fixation is not recommended, acetone fixation is preferred.

Reactivity

Mouse

Immunogen

Murine spleen cells

Concentration

1 mg/ml

Preparation

Purified by protein-G affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Ly-2, Ly-B, Ly-35, Lyt-2, BB154331

Antigen description

The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Entrez Gene ID 12525

UniProt ID P01731

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) purified antibody (concentration in sample 3 μg/ml) DAR PE.

Separation of murine CD8a positive splenocytes (red-filled) from myeloid cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD8a (53-6.7) purified antibody (concentration in sample 3 μg/ml) DAR PE.

Product specific references:

Mochimaru H, Usui T, Yaguchi T, Nagahama Y, Hasegawa G, Usui Y, Shimmura S, Tsubota K, Amano S, Kawakami Y, Ishida S: Suppression of alkali burn-induced corneal neovascularization by dendritic cell vaccination targeting VEGF receptor 2. Invest Ophthalmol Vis Sci. 2008 May;49(5):2172-7.
PubMed

Bouwer HG, Alberti-Segui C, Montfort MJ, Berkowitz ND, Higgins DE: Directed antigen delivery as a vaccine strategy for an intracellular bacterial pathogen. Proc Natl Acad Sci U S A. 2006 Mar 28;103(13):5102-7.
PubMed

Kamimura D, Sawa Y, Sato M, Agung E, Hirano T, Murakami M: IL-2 in vivo activities and antitumor efficacy enhanced by an anti-IL-2 mAb. J Immunol. 2006 Jul 1;177(1):306-14.
PubMed

Kosy, Ko HJ, Chang WS, Park SH, Kweon MN, Kang CY: alpha-Galactosylceramide can act as a nasal vaccine adjuvant inducing protective immune responses against viral infection and tumor. J Immunol. 2005 Sep 1;175(5):3309-17.
PubMed

Grabbe S, Varga G, Beissert S, Steinert M, Pendl G, Seeliger S, Bloch W, Peters T, Schwarz T, Sunderkötter C, Scharffetter-Kochanek K: Beta2 integrins are required for skin homing of primed T cells but not for priming naive T cells. J Clin Invest. 2002 Jan;109(2):183-92.
PubMed

Hata H, Sakaguchi N, Yoshitomi H, Iwakura Y, Sekikawa K, Azuma Y, Kanai C, Moriizumi E, Nomura T, Nakamura T, Sakaguchi S. Distinct contribution of IL-6, TNF-alpha, IL-1, and IL-10 to T cell-mediated spontaneous autoimmune arthritis in mice. J Clin Invest. 2004 Aug;114(4):582-8.
PubMed

Takahashi K, Nakata M, Tanaka T, Adachi H, Nakauchi H, Yagita H, Okumura K: CD4 and CD8 regulate interleukin 2 responses of T cells. Proc Natl Acad Sci U S A. 1992 Jun 15;89(12):5557-61.
PubMed

Ledbetter JA, Rouse RV, Micklem HS, Herzenberg LA: T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280-95.
PubMed

Ledbetter JA, Herzenberg LA: Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63-90.
PubMed