Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD86 APC

Anti-Ms CD86 APC

Regulatory status

RUO

Antigen

CD86

Clone

GL-1

Format

APC

Reactivity

Mouse

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

0.1 mg

1A-628-C100

Ask for delivery term HERE

209.00 USD

Variant

0.1 mg

1A-628-C100

Ask for delivery term HERE

209.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody GL-1 reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml; positive control: murine splenocytes.

Reactivity

Mouse

Immunogen

LPS-activated CBA/Cs mouse splenic B cells

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

B7-2, FUN-1, Ly58

Antigen description

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.

Entrez Gene ID 12524

UniProt ID P42082

Flow cytometry surface staining pattern of murine peritoneal fluid stained using anti-mouse CD86 (GL-1) APC antibody (concentration in sample 0,5 μg/ml).

Separation of murine CD86 positive monocytes (red-filled) from murine lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid stained using anti-mouse CD86 (GL-1) APC antibody (concentration in sample 0,5 μg/ml).

Product specific references:

Steptoe RJ, Ritchie JM, Jones LK, Harrison LC: Autoimmune diabetes is suppressed by transfer of proinsulin-encoding Gr-1+ myeloid progenitor cells that differentiate in vivo into resting dendritic cells. Diabetes. 2005 Feb;54(2):434-42.
PubMed

Nolan A, Kobayashi H, Naveed B, Kelly A, Hoshino Y, Hoshino S, Karulf MR, Rom WN, Weiden MD, Gold JA: Differential role for CD80 and CD86 in the regulation of the innate immune response in murine polymicrobial sepsis. PLoS One. 2009 Aug 12;4(8):e6600.
PubMed

Brasel K, De Smedt T, Smith JL, Maliszewski CR: Generation of murine dendritic cells from flt3-ligand-supplemented bone marrow cultures. Blood. 2000 Nov 1;96(9):3029-39.
PubMed

Nolan A, Weiden M, Kelly A, Hoshino Y, Hoshino S, Mehta N, Gold JA: CD40 and CD80/86 act synergistically to regulate inflammation and mortality in polymicrobial sepsis. Am J Respir Crit Care Med. 2008 Feb 1;177(3):301-8.
PubMed

Edgtton KL, Kausman JY, Li M, O'Sullivan K, Lo C, Hutchinson P, Yagita H, Holdsworth SR, Kitching AR: Intrarenal antigens activate CD4+ cells via co-stimulatory signals from dendritic cells. J Am Soc Nephrol. 2008 Mar;19(3):515-26.
PubMed

Hathcock KS, Laszlo G, Dickler HB, Bradshaw J, Linsley P, Hodes RJ: Identification of an alternative CTLA-4 ligand costimulatory for T cell activation. Science. 1993 Nov 5;262(5135):905-7.
PubMed

Benschop RJ, Melamed D, Nemazee D, Cambier JC: Distinct signal thresholds for the unique antigen receptor-linked gene expression programs in mature and immature B cells. J Exp Med. 1999 Sep 20;190(6):749-56.
PubMed

Radhakrishnan S, Arneson LN, Upshaw JL, Howe CL, Felts SJ, Colonna M, Leibson PJ, Rodriguez M, Pease LR: TREM-2 mediated signaling induces antigen uptake and retention in mature myeloid dendritic cells. J Immunol. 2008 Dec 1;181(11):7863-72.
PubMed

Chung JB, Wells AD, Adler S, Jacob A, Turka LA, Monroe JG: Incomplete activation of CD4 T cells by antigen-presenting transitional immature B cells: implications for peripheral B and T cell responsiveness. J Immunol. 2003 Aug 15;171(4):1758-67.
PubMed