Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD80 Purified

Anti-Ms CD80 Purified

Regulatory status

RUO

Antigen

CD80

Clone

16-10A1

Format

Purified

Reactivity

Mouse, Dog

Application

IP, FC (QC tested), IHC-F

Variant

0.1 mg

11-627-C100

In stock

88.00 USD

Variant

0.1 mg

11-627-C100

In stock

88.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Armenian Hamster IgG2

Specificity

The Armenian hamster monoclonal antibody 16-10A1 reacts with an extracellular epitope of CD80 (B7-1), a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

Application

IP, FC (QC tested), IHC-F

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Mouse, Dog

Immunogen

CD80-transfected CHO cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

B7-1, BB1

Antigen description

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152). The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.

Entrez Gene ID 12519

UniProt ID Q00609

Flow cytometry surface staining pattern of murine peripheral whole blood stained using anti-mouse CD80 (16-10A1) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Separation of murine CD80 positive monocytes (red-filled) from CD80 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid cells stained using anti-mouse CD80 (16-10A1) purified antibody (concentration in sample 2 µg/ml) GAM APC.

Product specific references:

Horspool JH, Perrin PJ, Woodcock JB, Cox JH, King CL, June CH, Harlan DM, St Louis DC, Lee KP: Nucleic acid vaccine-induced immune responses require CD28 costimulation and are regulated by CTLA4. J Immunol. 1998 Mar 15;160(6):2706-14.
PubMed

Mencacci A, Montagnoli C, Bacci A, Cenci E, Pitzurra L, Spreca A, Kopf M, Sharpe AH, Romani L: CD80+Gr-1+ myeloid cells inhibit development of antifungal Th1 immunity in mice with candidiasis. J Immunol. 2002 Sep 15;169(6):3180-90.
PubMed

Odobasic D, Kitching AR, Semple TJ, Timoshanko JR, Tipping PG, Holdsworth SR: Glomerular expression of CD80 and CD86 is required for leukocyte accumulation and injury in crescentic glomerulonephritis. J Am Soc Nephrol. 2005 Jul;16(7):2012-22.
PubMed

Reiser J, von Gersdorff G, Loos M, Oh J, Asanuma K, Giardino L, Rastaldi MP, Calvaresi N, Watanabe H, Schwarz K, Faul C, Kretzler M, Davidson A, Sugimoto H, Kalluri R, Sharpe AH, Kreidberg JA, Mundel P: Induction of B7-1 in podocytes is associated with nephrotic syndrome. J Clin Invest. 2004 May;113(10):1390-7.
PubMed

Paust S, Lu L, McCarty N, Cantor H: Engagement of B7 on effector T cells by regulatory T cells prevents autoimmune disease. Proc Natl Acad Sci U S A. 2004 Jul 13;101(28):10398-403.
PubMed

Quah B, Ni K, O'Neill HC: In vitro hematopoiesis produces a distinct class of immature dendritic cells from spleen progenitors with limited T cell stimulation capacity. Int Immunol. 2004 Apr;16(4):567-77.
PubMed

Mukherjee S, Ahmed A, Malu S, Nandi D: Modulation of cell cycle progression by CTLA4-CD80/CD86 interactions on CD4+ T cells depends on strength of the CD3 signal: critical role for IL-2. J Leukoc Biol. 2006 Jul;80(1):66-74.
PubMed

Gueirard P, Ave P, Balazuc AM, Thiberge S, Huerre M, Milon G, Guiso N: Bordetella bronchiseptica persists in the nasal cavities of mice and triggers early delivery of dendritic cells in the lymph nodes draining the lower and upper respiratory tract. Infect Immun. 2003 Jul;71(7):4137-43.
PubMed