Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD62L Purified Low Endotoxin

Anti-Ms CD62L Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD62L

Clone

Mel-14

Format

Purified Low Endotoxin

Reactivity

Mouse

Application

IP, FC (QC tested), IHC(F), IHC(P), ICC, FUNC

Variant

0.1 mg

12-626-C100

In stock

120.00 USD

Variant

0.1 mg

12-626-C100

In stock

120.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a

Specificity

The rat monoclonal antibody MEL-14 reacts with an extracellular epitope of murine CD62L (L-selectin), a 75 kDa single chain type I glycoprotein expressed on most peripheral blood B lymphocytes, T lymphocytes, monocytes and granulocytes; it is also present on a subset of NK cells and certain hematopoietic malignant cells.

Application

IP, FC (QC tested), IHC(F), IHC(P), ICC, FUNC

Application details

Functional application: Blocking of cell adhesion.
Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Mouse

Immunogen

C3H/eb mouse B cell lymphoma 38C-13

Concentration

1 mg/ml

Preparation

Purified by protein-G affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

L-selectin, Lsel, Sell, Lam1, Lecam, Leu8, Lnhr, Lyam1, Plnhr

Antigen description

CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1).

Entrez Gene ID 20343

UniProt ID Q3TCF3

Flow cytometry surface staining pattern of murine splenocytes stained using anti-mouse CD62L (Mel-14) purified antibody (concentration in sample 4 μg/ml, DAR APC).

Separation of murine splenocytes stained anti-mouse CD62L (Mel-14) purified antibody (concentration in sample 4 μg/ml, DAR APC, red-filled) from murine splenocytes unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Richards H, Longhi MP, Wright K, Gallimore A, Ager A: CD62L (L-selectin) down-regulation does not affect memory T cell distribution but failure to shed compromises anti-viral immunity. J Immunol. 2008 Jan 1;180(1):198-206.
PubMed

Chen BJ, Cui X, Sempowski GD, Liu C, Chao NJ: Transfer of allogeneic CD62L- memory T cells without graft-versus-host disease. Blood. 2004 Feb 15;103(4):1534-41.
PubMed

Xu H, Manivannan A, Crane I, Dawson R, Liversidge J: Critical but divergent roles for CD62L and CD44 in directing blood monocyte trafficking in vivo during inflammation. Blood. 2008 Aug 15;112(4):1166-74.
PubMed

Friedline RH, Wong CP, Steeber DA, Tedder TF, Tisch R: L-selectin is not required for T cell-mediated autoimmune diabetes. J Immunol. 2002 Mar 15;168(6):2659-66.
PubMed

Gallatin WM, Weissman IL, Butcher EC: A cell-surface molecule involved in organ-specific homing of lymphocytes. Nature 1983 Jul 7;304:30-34.
PubMed

Brawand P, Cerottini JC, MacDonald HR: Hierarchal utilization of different T-cell receptor Vbeta gene segments in the CD8(+)-T-cell response to an immunodominant Moloney leukemia virus-encoded epitope in vivo. J Virol. 1999 Nov;73(11):9161-9.
PubMed

Fisson S, Darrasse-Jèze G, Litvinova E, Septier F, Klatzmann D, Liblau R, Salomon BL: Continuous activation of autoreactive CD4+ CD25+ regulatory T cells in the steady state. J Exp Med. 2003 Sep 1;198(5):737-46.
PubMed

ten Bruggencate SJ, Hillyer LM, Woodward BD: The proportion of CD45RA(+)CD62L(+) (quiescent-phenotype) T cells within the CD8(+) subset increases in advanced weight loss in the protein- or energy-deficient weanling mouse. J Nutr. 2001 Dec;131(12):3266-9.
PubMed

Mannering SI, Cheers C: Interleukin-2 and loss of immunity in experimental Mycobacterium avium infection. Infect Immun. 2002 Jan;70(1):27-35.
PubMed

Pop SM, Wong CP, He Q, Wang Y, Wallet MA, Goudy KS, Tisch R: The type and frequency of immunoregulatory CD4+ T-cells govern the efficacy of antigen-specific immunotherapy in nonobese diabetic mice. Diabetes. 2007 May;56(5):1395-402.
PubMed

Chin CS, Miller CH, Graham L, Parviz M, Zacur S, Patel B, Duong A, Bear HD: Bryostatin 1/ionomycin (B/I) ex vivo stimulation preferentially activates L-selectinlow tumor-sensitized lymphocytes. Int Immunol. 2004 Sep;16(9):1283-94.
PubMed