Anti-Ms CD54 Purified Low Endotoxin

Anti-Ms CD54 Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD54
Clone
YN1/1.7.4
Format
Purified Low Endotoxin
Reactivity
Mouse
Application
IP, WB, FC (QC tested), IHC(F), ICC, FUNC
Variant
0.1 mg
12-625-C100
In stock
Variant
0.1 mg
11-261-C100
In stock
Product details
References
Isotype
Rat IgG2b
Specificity
The rat monoclonal antibody YN1/1.7.4 reacts with CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Application
IP, WB, FC (QC tested), IHC(F), ICC, FUNC
Reactivity
Mouse
Immunogen
Mouse NS-1 cells
Other names
ICAM-1, BB2, P3.58
Concentration
1 mg/ml
Preparation
Purified by protein-G affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Product specific references:

Sasaki M, Elrod JW, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: CYP450 dietary inhibitors attenuate TNF-alpha-stimulated endothelial molecule expression and leukocyte adhesion. Am J Physiol Cell Physiol. 2004 Apr;286(4):C931-9.
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Salas A, Gironella M, Salas A, Soriano A, Sans M, Iovanna J, Piqué JM, Panés J: Nitric oxide supplementation ameliorates dextran sulfate sodium-induced colitis in mice. Lab Invest. 2002 May;82(5):597-607.
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Greicius G, Westerberg L, Davey EJ, Buentke E, Scheynius A, Thyberg J, Severinson E: Microvilli structures on B lymphocytes: inducible functional domains? Int Immunol. 2004 Feb;16(2):353-64.
PubMed
Ma Z, Sharp KA, Janmey PA, Finkel TH: Surface-anchored monomeric agonist pMHCs alone trigger TCR with high sensitivity. PLoS Biol. 2008 Feb;6(2):e43.
PubMed
Sasaki M, Ostanin D, Elrod JW, Oshima T, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: TNF-alpha -induced endothelial cell adhesion molecule expression is cytochrome P-450 monooxygenase dependent. Am J Physiol Cell Physiol. 2003 Feb;284(2):C422-8.
PubMed
Horley KJ, Carpenito C, Baker B, Takei F: Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J. 1989 Oct;8(10):2889-96.
PubMed
Burns AR, Takei F, Doerschuk CM: Quantitation of ICAM-1 expression in mouse lung during pneumonia. J Immunol. 1994 Oct 1;153(7):3189-98.
PubMed
Soriano A, Salas A, Salas A, Sans M, Gironella M, Elena M, Anderson DC, Piqué JM, Panés J: VCAM-1, but not ICAM-1 or MAdCAM-1, immunoblockade ameliorates DSS-induced colitis in mice. Lab Invest. 2000 Oct;80(10):1541-51.
PubMed
Barrett SP, Riordon A, Toh BH, Gleeson PA, van Driel IR: Homing and adhesion molecules in autoimmune gastritis. J Leukoc Biol. 2000 Feb;67(2):169-73
PubMed
Variant
0.1 mg
12-625-C100
In stock
Variant
0.1 mg
11-261-C100
In stock