Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD54 Purified Low Endotoxin

Anti-Ms CD54 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD54

Clone

YN1/1.7.4

Format

Purified Low Endotoxin

Reactivity

Mouse

Application

IP, WB, FC (QC tested), IHC(F), ICC, FUNC

Variant

0.1 mg

12-625-C100

Ask for delivery term HERE

132.00 USD

Variant

0.1 mg

12-625-C100

Ask for delivery term HERE

132.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2b

Specificity

The rat monoclonal antibody YN1/1.7.4 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Application

IP, WB, FC (QC tested), IHC(F), ICC, FUNC

Application details

Functional application: Blocking.
Flow cytometry: Recommended dilution: 1 μg/ml. Western blotting: Non-reducing conditions.
Immunohistochemistry (frozen sections): Acetone fixation.

Reactivity

Mouse

Immunogen

Mouse NS-1 cells

Concentration

1 mg/ml

Preparation

Purified by protein-G affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ICAM-1, BB2, P3.58

Antigen description

CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Entrez Gene ID 15894

UniProt ID P13597

Flow cytometry surface staining pattern of murine peritoneal fluid cells stained using anti-mouse CD54 (YN1/1.7.4) purified antibody (concentration in sample 4 μg/ml, DAR APC).

Separation of murine leukocytes (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of murine peritoneal fluid cells stained using anti-mouse CD54 (YN1/1.7.4) purified antibody (concentration in sample 4 μg/ml, DAR APC).

Product specific references:

Sasaki M, Elrod JW, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: CYP450 dietary inhibitors attenuate TNF-alpha-stimulated endothelial molecule expression and leukocyte adhesion. Am J Physiol Cell Physiol. 2004 Apr;286(4):C931-9.
PubMed

Salas A, Gironella M, Salas A, Soriano A, Sans M, Iovanna J, Piqué JM, Panés J: Nitric oxide supplementation ameliorates dextran sulfate sodium-induced colitis in mice. Lab Invest. 2002 May;82(5):597-607.
PubMed

Chin JE, Winterrowd GE, Hatfield CA, Brashler JR, Griffin RL, Vonderfecht SL, Kolbasa KP, Fidler SF, Shull KL, Krzesicki RF, Ready KA, Dunn CJ, Sly LM, Staite ND, Richards IM: Involvement of intercellular adhesion molecule-1 in the antigen-induced infiltration of eosinophils and lymphocytes into the airways in a murine model of pulmonary inflammation. Am J Respir Cell Mol Biol. 1998 Feb;18(2):158-67.
PubMed

Greicius G, Westerberg L, Davey EJ, Buentke E, Scheynius A, Thyberg J, Severinson E: Microvilli structures on B lymphocytes: inducible functional domains? Int Immunol. 2004 Feb;16(2):353-64.
PubMed

Ma Z, Sharp KA, Janmey PA, Finkel TH: Surface-anchored monomeric agonist pMHCs alone trigger TCR with high sensitivity. PLoS Biol. 2008 Feb;6(2):e43.
PubMed

Sasaki M, Ostanin D, Elrod JW, Oshima T, Jordan P, Itoh M, Joh T, Minagar A, Alexander JS: TNF-alpha -induced endothelial cell adhesion molecule expression is cytochrome P-450 monooxygenase dependent. Am J Physiol Cell Physiol. 2003 Feb;284(2):C422-8.
PubMed

Horley KJ, Carpenito C, Baker B, Takei F: Molecular cloning of murine intercellular adhesion molecule (ICAM-1). EMBO J. 1989 Oct;8(10):2889-96.
PubMed

Burns AR, Takei F, Doerschuk CM: Quantitation of ICAM-1 expression in mouse lung during pneumonia. J Immunol. 1994 Oct 1;153(7):3189-98.
PubMed

Soriano A, Salas A, Salas A, Sans M, Gironella M, Elena M, Anderson DC, Piqué JM, Panés J: VCAM-1, but not ICAM-1 or MAdCAM-1, immunoblockade ameliorates DSS-induced colitis in mice. Lab Invest. 2000 Oct;80(10):1541-51.
PubMed

Barrett SP, Riordon A, Toh BH, Gleeson PA, van Driel IR: Homing and adhesion molecules in autoimmune gastritis. J Leukoc Biol. 2000 Feb;67(2):169-73
PubMed