Anti-Ms CD5 Purified

Anti-Ms CD5 Purified
Regulatory status
RUO
Antigen
CD5
Clone
53-7.3
Format
Purified
Reactivity
Mouse
Application
IP, FC (QC tested), IHC(F), IHC(P)
Variant
0.1 mg
11-748-C100
In stock
80.00 USD

0.025 mg
11-748-C025
Ask for delivery term HERE
40.00 USD
Variant
0.1 mg
11-261-C100
In stock
80.00 USD

0.1 mg
11-261-C100
In stock
40.00 USD
Product details
Description
References
Isotype
Rat IgG2a kappa
Specificity
The rat monoclonal antibody 53-7.3 recognizes an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B-1 lymphocytes.
Application
IP, FC (QC tested), IHC(F), IHC(P)
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Mouse
Immunogen
murine thymus or spleen cells
Other names
T1, LEU1
Concentration
1 mg/ml
Preparation
Purified from cell culture supernatant by protein-G affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
T1, LEU1
Antigen description
CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells it seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies. Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (rheumatoid arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Entrez Gene ID 12507
UniProt ID P13379

Product specific references:

Wang JY, Lee J, Yan M, Rho JH, Roehrl MH: Dermatan sulfate interacts with dead cells and regulates CD5(+) B-cell fate: implications for a key role in autoimmunity. Am J Pathol. 2011 May;178(5):2168-76.
PubMed
Sestero CM, McGuire DJ, De Sarno P, Brantley EC, Soldevila G, Axtell RC, Raman C: CD5-dependent CK2 activation pathway regulates threshold for T cell anergy. J Immunol. 2012 Sep 15;189(6):2918-30
PubMed
Jeong YI, Hong SH, Cho SH, Lee WJ, Lee SE: Induction of IL-10-producing CD1dhighCD5+ regulatory B cells following Babesia microti-infection. PLoS One. 2012;7(10):e46553. doi: 10.1371/journal.pone.0046553.
PubMed
Variant
0.1 mg
11-748-C100
In stock
80.00 USD

0.025 mg
11-748-C025
Ask for delivery term HERE
40.00 USD
Variant
0.1 mg
11-261-C100
In stock
80.00 USD

0.1 mg
11-261-C100
In stock
40.00 USD