Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD49e Purified

Anti-Ms CD49e Purified

Regulatory status

RUO

Antigen

CD49e

Clone

5H10-27 (MFR5)

Format

Purified

Reactivity

Mouse

Application

FC (QC tested), IHC(F), ICC

Variant

0.1 mg

11-743-C100

Ask for delivery term HERE

88.00 USD

0.025 mg

11-743-C025

Delivery 1 week

44.00 USD

Variant

0.1 mg

11-743-C100

Ask for delivery term HERE

88.00 USD

0.025 mg

11-743-C025

Delivery 1 week

44.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.

Application

FC (QC tested), IHC(F), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Mouse

Immunogen

C57BL/6 x A/J)F1 murine mast cell line

Concentration

1 mg/ml

Preparation

Purified by protein-G affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

ITGA5, VLA-5 alpha, FNRA

Antigen description

CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.

Entrez Gene ID 16402

UniProt ID P11688

Flow cytometry surface staining pattern of murine bone marrow using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 μg/ml, DAR APC).

Separation of murine bone marrow cells stained using anti-mouse CD49e (5H10-27(MFR5)) purified antibody (concentration in sample 0.56 μg/ml, DAR APC) from murine bone marrow cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

van der Loo JC, Xiao X, McMillin D, Hashino K, Kato I, Williams DA: VLA-5 is expressed by mouse and human long-term repopulating hematopoietic cells and mediates adhesion to extracellular matrix protein fibronectin. J Clin Invest. 1998 Sep 1;102(5):1051-61.
PubMed

Sampaio AL, Zahn G, Leoni G, Vossmeyer D, Christner C, Marshall JF, Perretti M: Inflammation-dependent alpha 5 beta 1 (very late antigen-5) expression on leukocytes reveals a functional role for this integrin in acute peritonitis. J Leukoc Biol. 2010 May;87(5):877-84.
PubMed

Plett PA, Frankovitz SM, Orschell CM: Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation. Blood. 2003 Sep 15;102(6):2285-91.
PubMed