Anti-Ms CD49e PE

Anti-Ms CD49e PE
Regulatory status
RUO
Antigen
CD49e
Clone
5H10-27 (MFR5)
Format
PE
Reactivity
Mouse
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-743-C100
In stock
132.00 USD
Variant
0.1 mg
1P-743-C100
In stock
132.00 USD
Product details
Description
References
SDS download
Isotype
Rat IgG2a kappa
Specificity
The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
Application
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Mouse
Immunogen
C57BL/6 x A/J)F1 murine mast cell line
Concentration
0.5 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
ITGA5, VLA-5 alpha, FNRA
Antigen description
CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.
Entrez Gene ID 16402
UniProt ID P11688

Product specific references:

van der Loo JC, Xiao X, McMillin D, Hashino K, Kato I, Williams DA: VLA-5 is expressed by mouse and human long-term repopulating hematopoietic cells and mediates adhesion to extracellular matrix protein fibronectin. J Clin Invest. 1998 Sep 1;102(5):1051-61.
PubMed
Sampaio AL, Zahn G, Leoni G, Vossmeyer D, Christner C, Marshall JF, Perretti M: Inflammation-dependent alpha 5 beta 1 (very late antigen-5) expression on leukocytes reveals a functional role for this integrin in acute peritonitis. J Leukoc Biol. 2010 May;87(5):877-84.
PubMed
Plett PA, Frankovitz SM, Orschell CM: Distribution of marrow repopulating cells between bone marrow and spleen early after transplantation. Blood. 2003 Sep 15;102(6):2285-91.
PubMed
Variant
0.1 mg
1P-743-C100
In stock
132.00 USD
Variant
0.1 mg
1P-743-C100
In stock
132.00 USD

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