Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD49d PE

Anti-Ms CD49d PE

Regulatory status

RUO

Antigen

CD49d

Clone

R1-2

Format

Reactivity

Mouse

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-734-C100

Ask for delivery term HERE

132.00 USD

Variant

0.1 mg

1P-734-C100

Ask for delivery term HERE

132.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2b kappa

Specificity

The rat monoclonal antibody R1-2 recognizes an extracellular epitope of murine CD49d / Integrin alpha 4 (VLA-4 alpha), an approximately 150 kDa glycoprotein of the integrin family, expressed on multiple blood cell types.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Mouse

Immunogen

murine T lymphoma line TK1

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Itga4, Vla-4 alpha

Antigen description

CD49d (integrin alpha 4), also known as very late antigen 4 alpha (VLA-4 alpha) is a cell surface glycoprotein constituting integrin complexes. CD49d heterodimerizes with CD29 (integrin beta 1) to form VLA-4 antigen, which is involved in cell-cell and cell-extracellular matrix interactions, which is a receptor for CD106 (VCAM) and fibronectin. CD79d also heterodimerizes with integrin beta 7 to form LPAM-1, which binds to MAdCAM-1 (mucosal vascular addressin). These interactions are important for cell adhesion and activation.

Entrez Gene ID 16401

UniProt ID Q00651

Flow cytometry analysis surface staining pattern of murine splenocyte suspension stained using anti-mouse CD49d (R1-2) PE antibody (concentration in sample 5 μg/ml).

Separation of murine CD49d positive cells (red-filled) from unstained sample (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD49d (R1-2) PE antibody (concentration in sample 5 μg/ml).

Product specific references:

Zhu X, Nishimura F, Sasaki K, Fujita M, Dusak JE, Eguchi J, Fellows-Mayle W, Storkus WJ, Walker PR, Salazar AM, Okada H: Toll like receptor-3 ligand poly-ICLC promotes the efficacy of peripheral vaccinations with tumor antigen-derived peptide epitopes in murine CNS tumor models. J Transl Med. 2007 Feb 12;5:10.
PubMed

Sadahira Y, Yasuda T, Yoshino T, Manabe T, Takeishi T, Kobayashi Y, Ebe Y, Naito M: Impaired splenic erythropoiesis in phlebotomized mice injected with CL2MDP-liposome: an experimental model for studying the role of stromal macrophages in erythropoiesis. J Leukoc Biol. 2000 Oct;68(4):464-70.
PubMed

Cheung DS, Ehlenbach SJ, Kitchens RT, Riley DA, Thomas LL, Holtzman MJ, Grayson MH: Cutting edge: CD49d+ neutrophils induce FcepsilonRI expression on lung dendritic cells in a mouse model of postviral asthma. J Immunol. 2010 Nov 1;185(9):4983-7.
PubMed

Geutskens SB, Nikolic T, Dardenne M, Leenen PJ, Savino W: Defective up-regulation of CD49d in final maturation of NOD mouse macrophages. Eur J Immunol. 2004 Dec;34(12):3465-76.
PubMed

Lin J, Qin L, Chavin KD, Ding Y, Punch JD, Yang Q, Burkly LC, Bromberg JS: CD3 and CD2 ligation alters CD49d epitope expression. Pathobiology. 1995;63(3):119-32.
PubMed