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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Ms CD45R PE
Anti-Ms CD45R PE
Regulatory status
RUO
Antigen
CD45R
Clone
RA3-6B2
Format
PE
Reactivity
Cat, Mouse, Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-715-C100
Out of stock
70.00 USD
0.025 mg
1P-715-C025
Out of stock
35.00 USD
Variant
0.1 mg
11-261-C100
In stock
70.00 USD
0.1 mg
11-261-C100
In stock
35.00 USD
Contact distributor
Product details
Images
References
Isotype
Rat IgG2a kappa
Specificity
The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml.
Reactivity
Cat, Mouse, Human
Immunogen
Abelson murine leukemia virus-induced pre-B tumor cells
Other names
PTPRCR, T200R
Concentration
0.5 mg/ml
Preparation
The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Flow cytometry analysis (surface staining) of CD45R in murine splenocytes with anti-CD45R (RA3-6B2) PE.
Product specific references:
Watanabe Y, Akaike T: Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells. Scand J Immunol. 1994 May;39(5):419-25.
PubMed
Tsurui H, Nishimura H, Hattori S, Hirose S, Okumura K, Shirai T: Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition. J Histochem Cytochem. 2000 May;48(5):653-62.
PubMed
Rada C, Ehrenstein MR, Neuberger MS, Milstein C: Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting. Immunity. 1998 Jul;9(1):135-41.
PubMed
Rothaeusler K, Baumgarth N: Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry. Cytometry A. 2006 Apr;69(4):249-59.
PubMed
Montecino-Rodriguez E, Leathers H, Dorshkind K: Identification of a B-1 B cell-specified progenitor. Nat Immunol. 2006 Mar;7(3):293-301.
PubMed
Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I: Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004 Apr 1;172(7):4298-307.
PubMed
Lochhead RB, Sonderegger FL, Ma Y, Brewster JE, Cornwall D, Maylor-Hagen H, Miller JC, Zachary JF, Weis JH, Weis JJ: Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue. J Immunol. 2012 Sep 1;189(5):2488-501
PubMed
Variant
0.1 mg
1P-715-C100
Out of stock
70.00 USD
0.025 mg
1P-715-C025
Out of stock
35.00 USD
Variant
0.1 mg
11-261-C100
In stock
70.00 USD
0.1 mg
11-261-C100
In stock
35.00 USD
Contact distributor
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