Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD45R APC

Anti-Ms CD45R APC

Regulatory status

RUO

Antigen

CD45R

Clone

RA3-6B2

Format

APC

Reactivity

Cat, Mouse, Human

Application

FC (QC tested)

Excitation laser

red (633 nm)

Variant

0.1 mg

1A-715-C100

In stock

132.00 USD

0.025 mg

1A-715-C025

Delivery 1 week

66.00 USD

Variant

0.1 mg

1A-715-C100

In stock

132.00 USD

0.025 mg

1A-715-C025

Delivery 1 week

66.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody RA3-6B2 recognizes an extracellular epitope on CD45R, which is expressed at all developmental stages of B cells, including activated B cells, but also on subsets of NK and T cells. T cells detected by this antibody are supposed to be in activated state.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Cat, Mouse, Human

Immunogen

Abelson murine leukemia virus-induced pre-B tumor cells

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PTPRCR, T200R

Antigen description

CD45R, also known as B220, is a receptor-type protein tyrosine phosphatase glycoprotein. It is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45R expression also identifies a subset of murine bone marrow cells able to form osteoclast-like cells.

Flow cytometry analysis (surface staining) of murine splenocytes using anti-mouse CD45R (RA3-6B2)APC antibody (red, concentration in sample 1 μg/ml) with blank sample (blue).

Product specific references:

Watanabe Y, Akaike T: Activation signal induces the expression of B cell-specific CD45R epitope (6B2) on murine T cells. Scand J Immunol. 1994 May;39(5):419-25.
PubMed

Tsurui H, Nishimura H, Hattori S, Hirose S, Okumura K, Shirai T: Seven-color fluorescence imaging of tissue samples based on Fourier spectroscopy and singular value decomposition. J Histochem Cytochem. 2000 May;48(5):653-62.
PubMed

Rada C, Ehrenstein MR, Neuberger MS, Milstein C: Hot spot focusing of somatic hypermutation in MSH2-deficient mice suggests two stages of mutational targeting. Immunity. 1998 Jul;9(1):135-41.
PubMed

Rothaeusler K, Baumgarth N: Evaluation of intranuclear BrdU detection procedures for use in multicolor flow cytometry. Cytometry A. 2006 Apr;69(4):249-59.
PubMed

Montecino-Rodriguez E, Leathers H, Dorshkind K: Identification of a B-1 B cell-specified progenitor. Nat Immunol. 2006 Mar;7(3):293-301.
PubMed

Cappione AJ, Pugh-Bernard AE, Anolik JH, Sanz I: Lupus IgG VH4.34 antibodies bind to a 220-kDa glycoform of CD45/B220 on the surface of human B lymphocytes. J Immunol. 2004 Apr 1;172(7):4298-307.
PubMed

Lochhead RB, Sonderegger FL, Ma Y, Brewster JE, Cornwall D, Maylor-Hagen H, Miller JC, Zachary JF, Weis JH, Weis JJ: Endothelial cells and fibroblasts amplify the arthritogenic type I IFN response in murine Lyme disease and are major sources of chemokines in Borrelia burgdorferi-infected joint tissue. J Immunol. 2012 Sep 1;189(5):2488-501
PubMed