Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD44 PE-Cy™7

Anti-Ms CD44 PE-Cy™7

Regulatory status

RUO

Antigen

CD44

Clone

IM7

Format

PE-Cy™7

Reactivity

Dog, Mouse, Human, Cat, Horse

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

T7-624-C100

In stock

198.00 USD

0.025 mg

T7-624-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

T7-624-C100

In stock

198.00 USD

0.025 mg

T7-624-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2b kappa

Specificity

The rat monoclonal antibody IM7 reacts with an extracellular epitope of CD44 antigen (Phagocyte glycoprotein 1), an 80-95 kDa transmembrane glycoprotein (hyaladherin family) present on the most of cells and tissues (leukocytes, endothelial cells, mesenchymal cells, etc.); it is negative on platelets and hepatocytes. The antibody reacts with all isoforms of mouse CD44.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-3 μg/ml.

Reactivity

Dog, Mouse, Human, Cat, Horse

Immunogen

Dexamethasone-induced cells of the SJL mouse spontaneous myeloid leukemia M1

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

PGP-I, HUTCH-I, ECMR-III, Hermes antigen, Hyaluronate receptor, Heparan sulfate proteoglycan, Epican, MC56, MIC4

Antigen description

CD44 is a transmembrane glycoprotein expressed on the surface of most cells, which serves as a receptor for hyaluronan. CD44 mediates angiogenesis, cell adhesion, proliferation and migration, it is thus important for lymphocyte activation, recirculation and homing, it can thus serve e.g. as a modulator of macrophage recruitment in response to pathogen. Although CD44 functions are essential for physiological activities of normal cells, elevated CD44 expression correlates with poor prognosis in many carcinomas, facilitating tumour growth and metastasis, antiapoptosis and directional motility of cancer cells.

Entrez Gene ID 12505

UniProt ID P15379

Flow cytometry analysis (surface staining) of murine splenocytes with anti-CD44 (IM7) PE-Cy™7.

Product specific references:

Lesley J, Trowbridge IS: Genetic characterization of a polymorphic murine cell-surface glycoprotein. Immunogenetics. 1982 Mar;15(3):313-20.
PubMed

Hegde VL, Singh NP, Nagarkatti PS, Nagarkatti M: CD44 mobilization in allogeneic dendritic cell-T cell immunological synapse plays a key role in T cell activation. J Leukoc Biol. 2008 Jul;84(1):134-42.
PubMed

van Royen N, Voskuil M, Hoefer I, Jost M, de Graaf S, Hedwig F, Andert JP, Wormhoudt TA, Hua J, Hartmann S, Bode C, Buschmann I, Schaper W, van der Neut R, Piek JJ, Pals ST: CD44 regulates arteriogenesis in mice and is differentially expressed in patients with poor and good collateralization. Circulation. 2004 Apr 6;109(13):1647-52.
PubMed

McKallip RJ, Do Y, Fisher MT, Robertson JL, Nagarkatti PS, Nagarkatti M: Role of CD44 in activation-induced cell death: CD44-deficient mice exhibit enhanced T cell response to conventional and superantigens. Int Immunol. 2002 Sep;14(9):1015-26.
PubMed

Katoh S, Matsumoto N, Kawakita K, Tominaga A, Kincade PW, Matsukura S: A role for CD44 in an antigen-induced murine model of pulmonary eosinophilia. J Clin Invest. 2003 May;111(10):1563-70.
PubMed

Legg JW, Lewis CA, Parsons M, Ng T, Isacke CM: A novel PKC-regulated mechanism controls CD44 ezrin association and directional cell motility. Nat Cell Biol. 2002 Jun;4(6):399-407.
PubMed

Si-Tahar M, Sitaraman S, Shibahara T, Madara JL: Negative regulation of epithelium-neutrophil interactions via activation of CD44. Am J Physiol Cell Physiol. 2001 Mar;280(3):C423-32.
PubMed

Larkin J, Renukaradhya GJ, Sriram V, Du W, Gervay-Hague J, Brutkiewicz RR: CD44 differentially activates mouse NK T cells and conventional T cells. J Immunol. 2006 Jul 1;177(1):268-79.
PubMed

Xu H, Manivannan A, Liversidge J, Sharp PF, Forrester JV, Crane IJ: Involvement of CD44 in leukocyte trafficking at the blood-retinal barrier. J Leukoc Biol. 2002 Dec;72(6):1133-41.
PubMed

Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed