Anti-Ms CD41 Purified Low Endotoxin

Anti-Ms CD41 Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD41
Clone
MWReg30
Format
Purified Low Endotoxin
Reactivity
Mouse
Variant
0.1 mg
12-763-C100
In stock
100.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD
Product details
Description
Images
References
Isotype
Rat IgG1 kappa
Specificity
The rat monoclonal antibody MWReg30 recognizes an extracellular epitope of CD41 (GPIIb), a transmembrane glycoprotein (integrin family) composed of two chains GPIIb alpha (heavy chain; 120 kDa) and GPIIb beta (light chain; 23 kDa). CD41 is mainly expressed on platelets and megakaryocytes.
Application details
Functional application: Platelet depletion.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Mouse
Immunogen
Murine platelets
Concentration
1 mg/ml
Preparation
Purified by protein-G affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Platelet GPIIb, Integrin alpha-IIb, GPalpha IIb, GPIIb
Antigen description
CD41 (platelet glycoprotein IIb) is composed of two subunits (120 kDa a, alpha and 23 kDa b, beta) that interact with CD61 in the presence of calcium to form a functional adhesive protein receptor. Upon blood vessel damage, this receptor binds to a variety of proteins including von Willebrand factor, fibrinogen, fibronectin and vitronectin. CD41 is mainly expressed on megakaryocyte-platelet lineage, but generally belongs to the antigens that are expressed during early stages of hematopoietic differentiation.
Entrez Gene ID 16399
UniProt ID Q9QUM0
12-763_FC_Profil Značení
Flow cytometry surface staining pattern of murine splenocytes stained using anti-mouse CD41 (MWReg30) purified antibody (low endotoxin, concentration in sample 0,6 μg/ml, DAR APC).
12-763_FC_Histogram
Separation of murine CD41 negative splenocytes (red-filled) from CD41 positive thrombocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD41 (MWReg30) purified antibody (low endotoxin, concentration in sample 0,6 μg/ml, DAR APC).

Product specific references:

Ramsey H, Zhang Q, Brown DE, Steensma DP, Lin CP, Wu MX: Stress-induced hematopoietic failure in the absence of immediate early response gene X-1. Haematologica. 2013 Sep 20. [Epub ahead of print]
PubMed
Sullivan BP, Wang R, Tawfik O, Luyendyk JP: Protective and damaging effects of platelets in acute cholestatic liver injury revealed by depletion and inhibition strategies. Toxicol Sci. 2010 May;115(1):286-94.
PubMed
Boisset JC, Clapes T, Van Der Linden R, Dzierzak E, Robin C: Integrin αIIb (CD41) plays a role in the maintenance of hematopoietic stem cell activity in the mouse embryonic aorta. Biol Open. 2013 Apr 11;2(5):525-32.
PubMed
Brancaleone V, Gobbetti T, Cenac N, le Faouder P, Colom B, Flower RJ, Vergnolle N, Nourshargh S, Perretti M: A vasculo-protective circuit centered on lipoxin A4 and aspirin-triggered 15-epi-lipoxin A4 operative in murine microcirculation. Blood. 2013 Jul 25;122(4):608-17.
PubMed
Variant
0.1 mg
12-763-C100
In stock
100.00 USD
Variant
0.1 mg
11-261-C100
In stock
100.00 USD