Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD4 FITC

Anti-Ms CD4 FITC

Regulatory status

RUO

Antigen

CD4

Clone

GK1.5

Format

FITC

Reactivity

Mouse

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1F-580-C100

In stock

110.00 USD

Variant

0.1 mg

1F-580-C100

In stock

110.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2b

Specificity

The rat monoclonal antibody GK1.5 reacts with an extracellular epitope of mouse CD4 transmembrane glycoprotein (55 kDa).

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-2 μg/ml.

Reactivity

Mouse

Immunogen

Mouse CTL clone V4 cells

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

T4/Leu-3, L3T4

Antigen description

CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).

Entrez Gene ID 12504

UniProt ID P06332

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) FITC antibody (concentration in sample 0,33 μg/ml).

Separation of murine CD4 positive cells (red-filled) from murine CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD4 (GK1.5) FITC antibody (concentration in sample 0,33 μg/ml).

Product specific references:

Yi H, Zhen Y, Zeng C, Zhang L, Zhao Y: Depleting anti-CD4 monoclonal antibody (GK1.5) treatment: influence on regulatory CD4+CD25+Foxp3+ T cells in mice. Transplantation. 2008 Apr 27;85(8):1167-74.
PubMed

Hu M, Watson D, Zhang GY, Graf N, Wang YM, Sartor M, Howden B, Fletcher J, Alexander SI: Long-term cardiac allograft survival across an MHC mismatch after "pruning" of alloreactive CD4 T cells. J Immunol. 2008 May 15;180(10):6593-603.
PubMed

Felix NJ, Donermeyer DL, Horvath S, Walters JJ, Gross ML, Suri A, Allen PM: Alloreactive T cells respond specifically to multiple distinct peptide-MHC complexes. Nat Immunol. 2007 Apr;8(4):388-97.
PubMed

Zheng B, Han S, Kelsoe G: T helper cells in murine germinal centers are antigen-specific emigrants that downregulate Thy-1. J Exp Med. 1996 Sep 1;184(3):1083-91.
PubMed

Gavett SH, Chen X, Finkelman F, Wills-Karp M: Depletion of murine CD4+ T lymphocytes prevents antigen-induced airway hyperreactivity and pulmonary eosinophilia. Am J Respir Cell Mol Biol. 1994 Jun;10(6):587-93.
PubMed

Godfrey DI, Kennedy J, Gately MK, Hakimi J, Hubbard BR, Zlotnik A: IL-12 influences intrathymic T cell development. J Immunol. 1994 Mar 15;152(6):2729-35.
PubMed

Wu L, Antica M, Johnson GR, Scollay R, Shortman K: Developmental potential of the earliest precursor cells from the adult mouse thymus. J Exp Med. 1991 Dec 1;174(6):1617-27.
PubMed

Wilde DB, Marrack P, Kappler J, Dialynas DP, Fitch FW: Evidence implicating L3T4 in class II MHC antigen reactivity; monoclonal antibody GK1.5 (anti-L3T4a) blocks class II MHC antigen-specific proliferation, release of lymphokines, and binding by cloned murine helper T lymphocyte lines. J Immunol. 1983 Nov;131(5):2178-83.
PubMed

Dialynas DP, Quan ZS, Wall KA, Pierres A, Quintáns J, Loken MR, Pierres M, Fitch FW: Characterization of the murine T cell surface molecule, designated L3T4, identified by monoclonal antibody GK1.5: similarity of L3T4 to the human Leu-3/T4 molecule. J Immunol. 1983 Nov;131(5):2445-51.
PubMed

Dialynas DP, Wilde DB, Marrack P, Pierres A, Wall KA, Havran W, Otten G, Loken MR, Pierres M, Kappler J, et al.: Characterization of the murine antigenic determinant, designated L3T4a, recognized by monoclonal antibody GK1.5: expression of L3T4a by functional T cell clones appears to correlate primarily with class II MHC antigen-reactivity. Immunol Rev. 1983;74:29-56.
PubMed