Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD28 Purified Low Endotoxin

Anti-Ms CD28 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD28

Clone

37.51

Format

Purified Low Endotoxin

Reactivity

Mouse

Application

IP, FC (QC tested), IHC(F), ICC, FUNC

Variant

0.1 mg

12-597-C100

In stock

80.00 USD

Variant

0.1 mg

12-597-C100

In stock

80.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Golden Syrian Hamster IgG

Specificity

The Syrian hamster monoclonal antibody 37.51 reacts with extracellular domain of mouse CD28 costimulatory receptor, a 45 kDa homodimeric protein expressed by thymocytes, mature T cells and NK cells.

Application

IP, FC (QC tested), IHC(F), ICC, FUNC

Application details

Functional application: In vitro and in vivo T cell costimulation.
Flow cytometry: Recommended dilution: 6 μg / ml.
Immunocytochemistry: Recommended dilution: 1-4 μg / ml. Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.

Reactivity

Mouse

Immunogen

Mouse T cell lymphoma EL-4 cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

This product is for research and in vitro experimental use only. It is not to be used for any other commercial purpose. Use of this product to produce products for sale or for therapeutic or drug discovery purposes is prohibited. In order to obtain a license to use this product for commercial purposes, contact The Regents of the University of California.

Other names

Tp44

Antigen description

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Entrez Gene ID 12487

UniProt ID P31041

Flow cytometry analysis (surface staining) of murine peritoneal fluid cells using anti-CD28 (clone 37.51) purified, GAArH-FITC.

Product specific references:

Zhang et al.: The long isoform of cellular FLIP is essential for T lymphocyte proliferation through an NF-kappaB-independent pathway. J Immunol. 2008 Apr 15;180(8):5506-11.
PubMed

Rosenberger CM, Clark AE, Treuting PM, Johnson CD, Aderem A: ATF3 regulates MCMV infection in mice by modulating IFN-gamma expression in natural killer cells. Proc Natl Acad Sci U S A. 2008 Feb 19;105(7):2544-9.
PubMed

Perchonock CE, Pajerowski AG, Nguyen C, Shapiro MJ, Shapiro VS: The related adaptors, adaptor in lymphocytes of unknown function X and Rlk/Itk-binding protein, have nonredundant functions in lymphocytes. J Immunol. 2007 Aug 1;179(3):1768-75.
PubMed

Pua HH, Dzhagalov I, Chuck M, Mizushima N, He YW: A critical role for the autophagy gene Atg5 in T cell survival and proliferation. J Exp Med. 2007 Jan 22;204(1):25-31.
PubMed

Perchonock CE, Fernando MC, Quinn WJ 3rd, Nguyen CT, Sun J, Shapiro MJ, Shapiro VS: Negative regulation of interleukin-2 and p38 mitogen-activated protein kinase during T-cell activation by the adaptor ALX. Mol Cell Biol. 2006 Aug;26(16):6005-15.
PubMed

Zhang N, He YW: The antiapoptotic protein Bcl-xL is dispensable for the development of effector and memory T lymphocytes. J Immunol. 2005 Jun 1;174(11):6967-73.
PubMed

Zhang N, He YW: An essential role for c-FLIP in the efficient development of mature T lymphocytes. J Exp Med. 2005 Aug 1;202(3):395-404.
PubMed

Albert MH, Yu XZ, Martin PJ, Anasetti C: Prevention of lethal acute GVHD with an agonistic CD28 antibody and rapamycin. Blood. 2005 Feb 1;105(3):1355-61.
PubMed

Gross JA, Callas E, Allison JP: Identification and distribution of the costimulatory receptor CD28 in the mouse. J Immunol. 1992 Jul 15;149(2):380-8.
PubMed

Harding FA, McArthur JG, Gross JA, Raulet DH, Allison JP: CD28-mediated signalling co-stimulates murine T cells and prevents induction of anergy in T-cell clones. Nature. 1992 Apr 16;356(6370):607-9.
PubMed