Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD25 FITC

Anti-Ms CD25 FITC

Regulatory status

RUO

Antigen

CD25

Clone

PC61.5

Format

FITC

Reactivity

Mouse

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1F-614-C100

Ask for delivery term HERE

100.00 USD

Variant

0.1 mg

1F-614-C100

Ask for delivery term HERE

100.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG1 lambda

Specificity

The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes an extracellular epitope of CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4⁺ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-3 μg/ml.

Reactivity

Mouse

Immunogen

B6.1 CTL cell line

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

IL-2R α chain, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10

Antigen description

CD25 (IL2Ralpha, Tac) is a ligand-binding alpha subunit of interleukin 2 receptor (IL2R). Together with beta and gamma subunit CD25 constitues the high affinity IL2R, whereas CD25 alone serves as the low affinity IL2R. CD25 expression rapidly increases upon T cell activation. The 55 kDa CD25 molecule is enzymatically cleaved and shed from the cell surface as a soluble 45 kDa s-Tac, whose concentration in serum can be used as a marker of T cell activation. Expression of CD25 indicates the neoplastic phenotype of mast cells. Humanized anti CD25 antibodies represent a useful tool to reduce the incidence of allograft rejection as well as the severity of graft versus host reaction, and radioimmunoconjugates of anti-CD25 antibodies can be used against CD25 expressing lymphomas.

Entrez Gene ID 16184

UniProt ID P01590

Flow cytometry analysis (surface staining) of CD25 in PHA activated (3 days) murine splenocytes using anti-CD25 (PC61.5) FITC.

Product specific references:

Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J Biomed Biotechnol. 2010;2010:786078.
PubMed

Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice. J Immunol. 2008 Nov 15;181(10):7221-9.
PubMed

Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection. Mol Cell Biol. 2002 Jul;22(13):4556-66.
PubMed

Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997 Jun 1;89(11):4005-12.
PubMed

Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL-2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep;78(3):725-35.
PubMed

Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J Immunol. 2008 Oct 15;181(8):5204-8.
PubMed

Kotner J, Tarleton R: Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007 Feb;75(2):861-9.
PubMed

Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J Virol. 2000 Mar;74(5):2414-9.
PubMed