Anti-Ms CD25 APC

Anti-Ms CD25 APC
Regulatory status
RUO
Antigen
CD25
Clone
PC61.5
Format
APC
Reactivity
Mouse
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
0.1 mg
1A-614-C100
In stock

0.025 mg
1A-614-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Rat IgG1 lambda
Specificity
The rat monoclonal antibody PC61.5 (PC61.5.3) recognizes CD25 (Interleukin-2 receptor alpha chain), a 55 kDa type I transmembrane glycoprotein expressed on activated B and T lymphocytes, activated monocytes/macrophages and on CD4+ T lymphocytes (T regulatory cells); it is lost on resting B and T lymphocytes.
Application
FC (QC tested)
Reactivity
Mouse
Immunogen
B6.1 CTL cell line
Other names
IL-2R α chain, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2R subunit alpha, IL-2-RA, IL2-RA, p55, TAC antigen, IDDM10
Concentration
0.5 mg/ml
Preparation
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
1A-614 FC
Flow cytometry analysis (surface staining) of CD25 in PHA activated (3 days) murine splenocytes using anti-CD25 (PC61.5) APC.

Product specific references:

Tenorio EP, Olguín JE, Fernández J, Vieyra P, Saavedra R: Reduction of Foxp3+ cells by depletion with the PC61 mAb induces mortality in resistant BALB/c mice infected with Toxoplasma gondii. J Biomed Biotechnol. 2010;2010:786078.
PubMed
Wei HX, Chuang YH, Li B, Wei H, Sun R, Moritoki Y, Gershwin ME, Lian ZX, Tian Z: CD4+ CD25+ Foxp3+ regulatory T cells protect against T cell-mediated fulminant hepatitis in a TGF-beta-dependent manner in mice. J Immunol. 2008 Nov 15;181(10):7221-9.
PubMed
Yu CT, Feng MH, Shih HM, Lai MZ: Increased p300 expression inhibits glucocorticoid receptor-T-cell receptor antagonism but does not affect thymocyte positive selection. Mol Cell Biol. 2002 Jul;22(13):4556-66.
PubMed
Aiba Y, Hirayama F, Ogawa M: Clonal proliferation and cytokine requirement of murine progenitors for natural killer cells. Blood. 1997 Jun 1;89(11):4005-12.
PubMed
Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL-2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J Leukoc Biol. 2005 Sep;78(3):725-35.
PubMed
Van VQ, Darwiche J, Raymond M, Lesage S, Bouguermouh S, Rubio M, Sarfati M: Cutting edge: CD47 controls the in vivo proliferation and homeostasis of peripheral CD4+ CD25+ Foxp3+ regulatory T cells that express CD103. J Immunol. 2008 Oct 15;181(8):5204-8.
PubMed
Kotner J, Tarleton R: Endogenous CD4(+) CD25(+) regulatory T cells have a limited role in the control of Trypanosoma cruzi infection in mice. Infect Immun. 2007 Feb;75(2):861-9.
PubMed
Jones CM, Cose SC, Coles RM, Winterhalter AC, Brooks AG, Heath WR, Carbone FR: Herpes simplex virus type 1-specific cytotoxic T-lymphocyte arming occurs within lymph nodes draining the site of cutaneous infection. J Virol. 2000 Mar;74(5):2414-9.
PubMed
Variant
0.1 mg
1A-614-C100
In stock

0.025 mg
1A-614-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock