Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD2 Purified

Anti-Ms CD2 Purified

Regulatory status

RUO

Antigen

CD2

Clone

RM2-5

Format

Purified

Reactivity

Mouse

Application

IP, FC (QC tested)

Variant

0.1 mg

11-716-C100

In stock

90.20 USD

Variant

0.1 mg

11-716-C100

In stock

90.20 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2b lambda

Specificity

The rat monoclonal antibody RM2-5 recognizes an extracellular epitope of CD2, a 50 kDa glycoprotein present on the human peripheral blood T lymphocytes and NK cells; also expressed by all thymocytes.

Application

IP, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Mouse

Immunogen

murine thymocytes

Concentration

1 mg/ml

Preparation

Purified by protein-G affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

LFA-2, Ly37, Ly-37

Antigen description

CD2 belongs to T lymphocyte glycoproteins of immunoglobulin superfamily. Its interaction with CD58 stabilizes adhesion between T cells and antigen presenting or target cells. Relatively low affinity of CD2 to CD58 (as measured in solution) is compensated within the two-dimensional cell-cell interface to provide tight adhesion. Moreover, T cell activation induces increased CD2 expression and its lateral mobility, making easier contact between CD2 and CD58. Subsequently, T cell activation causes fixation of CD58-CD2 at sites of cell-cell contact, thereby strengthening intercellular adhesion. CD2 deficiency reduces intestinal inflammation and helps to control infection.

Entrez Gene ID 12481

UniProt ID P08920

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD2 (RM2-5) purified antibody (concentration in sample 0,44 μg/ml, GAM APC).

Separation of murine CD2 positive cells (red-filled) from CD2 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD2 (RM2-5) purified antibody (concentration in sample 0,44 μg/ml, GAM APC).

Product specific references:

Wallin J, Eibel H, Neubüser A, Wilting J, Koseki H, Balling R: Pax1 is expressed during development of the thymus epithelium and is required for normal T-cell maturation. Development. 1996 Jan;122(1):23-30.
PubMed

Altin JG, Pagler EB, Parish CR: Evidence for cell surface association of CD2 and LFA-1 (CD11a/CD18) on T lymphocytes. Eur J Immunol. 1994 Feb;24(2):450-7.
PubMed

Sasada T, Reinherz EL: A critical role for CD2 in both thymic selection events and mature T cell function. J Immunol. 2001 Feb 15;166(4):2394-403.
PubMed

Cho HJ, Edmondson SG, Miller AD, Sellars M, Alexander ST, Somersan S, Punt JA: Cutting edge: identification of the targets of clonal deletion in an unmanipulated thymus. J Immunol. 2003 Jan 1;170(1):10-3.
PubMed

Fassett MS, Jiang W, D'Alise AM, Mathis D, Benoist C: Nuclear receptor Nr4a1 modulates both regulatory T-cell (Treg) differentiation and clonal deletion. Proc Natl Acad Sci U S A. 2012 Mar 6; 109(10):3891-6.
PubMed

Milstein O, Tseng SY, Starr T, Llodra J, Nans A, Liu M, Wild MK, van der Merwe PA, Stokes DL, Reisner Y, Dustin ML: Nanoscale increases in CD2-CD48-mediated intermembrane spacing decrease adhesion and reorganize the immunological synapse. J Biol Chem. 2008 Dec 5;283(49):34414-22.
PubMed

Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wülfing C, Schatzle J, Mathew PA, Sharpe AH, Kumar V: Requirement of homotypic NK-cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions. Blood. 2006 Apr 15;107(8):3181-8.
PubMed

Cibotti R, Bhandoola A, Guinter TI, Sharrow SO, Singer A: CD8 coreceptor extinction in signaled CD4(+)CD8(+) thymocytes: coordinate roles for both transcriptional and posttranscriptional regulatory mechanisms in developing thymocytes. Mol Cell Biol. 2000 Jun;20(11):3852-9.
PubMed

Izon DJ, Punt JA, Xu L, Karnell FG, Allman D, Myung PS, Boerth NJ, Pui JC, Koretzky GA, Pear WS: Notch1 regulates maturation of CD4+ and CD8+ thymocytes by modulating TCR signal strength. Immunity. 2001 Mar;14(3):253-64.
PubMed