Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD197 PE

Anti-Ms CD197 PE

Regulatory status

RUO

Antigen

CD197

Clone

4B12

Format

Reactivity

Mouse

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-735-C100

In stock

198.00 USD

0.025 mg

1P-735-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

1P-735-C100

In stock

198.00 USD

0.025 mg

1P-735-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a kappa

Specificity

The rat monoclonal antibody 4B12 recognizes an extracellular epitope of murine CD197 / CCR7 (chemokine receptor 7), a 43 kDa G-protein-coupled receptor for chemokines CCL19 and CCL21.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Mouse

Immunogen

Murine CD197-transfected RBL-2H3 cells

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CCR7, CC-CKR-7, EBI1

Antigen description

CD197 / CCR7 (chemokine receptor 7) is a seven membrane-spanning protein serving as a receptor for chemokines CCL19 and CCL21. It is expressed on most naive T cells, some hematopoietic stem cells, mature dendritic cells, NK cells and some memory T cells and B cells subsets. CD197 plays important roles in development of thymocytes, and regulating the recirculation and homing of lymphocytes and dendritic cells to secondary lymphoid organs.

Entrez Gene ID 12775

UniProt ID P47774

Separation of murine splenocytes stained using anti-mouse CD197 (4B12) PE antibody (concentration in sample 9 μg/ml, red) from unstained murine splenocytes (black) in flow cytometry analysis (surface staining).

Product specific references:

Singh V, Gowthaman U, Jain S, Parihar P, Banskar S, Gupta P, Gupta UD, Agrewala JN: Coadministration of interleukins 7 and 15 with bacille Calmette-Guérin mounts enduring T cell memory response against Mycobacterium tuberculosis. J Infect Dis. 2010 Aug 15;202(3):480-9
PubMed

Adachi K, Osada Y, Nakamura R, Tamada K, Hamano S: Unique T cells with unconventional cytokine profiles induced in the livers of mice during schistosoma mansoni infection. PLoS One. 2013 Dec 16;8(12):e82698.
PubMed

Huggins A, Paschalidis N, Flower RJ, Perretti M, D'Acquisto F: Annexin-1-deficient dendritic cells acquire a mature phenotype during differentiation. FASEB J. 2009 Apr;23(4):985-96
PubMed

Eisenbarth SC, Williams A, Colegio OR, Meng H, Strowig T, Rongvaux A, Henao-Mejia J, Thaiss CA, Joly S, Gonzalez DG, Xu L, Zenewicz LA, Haberman AM, Elinav E, Kleinstein SH, Sutterwala FS, Flavell RA: NLRP10 is a NOD-like receptor essential to initiate adaptive immunity by dendritic cells. Nature. 2012 Apr 25;484(7395):510-3
PubMed