Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD19 Purified

Anti-Ms CD19 Purified

Anti-Ms CD19 Purified
Regulatory status
RUO
Antigen
CD19
Clone
1D3
Format
Purified
Reactivity
Mouse
Application
IP, FC (QC tested), IHC(F)
Variant
0.1 mg
11-596-C100
In stock
80.00 USD
0.025 mg
11-596-C025
Out of stock
40.00 USD
Variant
0.1 mg
11-261-C100
In stock
80.00 USD
0.1 mg
11-261-C100
In stock
40.00 USD
Contact distributor
Product details
Images
References
Isotype
Rat IgG2a
Specificity
The rat monoclonal antibody 1D3 detects an extracellular epitope of murine CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.
Application
IP, FC (QC tested), IHC(F)
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Mouse
Immunogen
Mouse CD19-transfected cell line
Other names
B4, Leu-12, Cvid
Concentration
1 mg/ml
Preparation
Purified by protein-G affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-596_FC_Profil Značení
Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD19 (1D3) purified antibody (concentration in sample 0,6 μg/ml) DAR APC.
11-596_FC_Histogram
Separation of murine CD19 positive splenocytes (red-filled) from CD19 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD19 (1D3) purified antibody (concentration in sample 0,6 μg/ml) DAR APC.

Product specific references:

Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S: The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment. J Immunol. 2003 Oct 15;171(8):4062-72.
PubMed
Inabe K, Kurosaki T: Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99(2):584-9.
PubMed
Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT: Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan;26(1):238-42.
PubMed
Cherukuri A, Cheng PC, Pierce SK: The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens. J Immunol. 2001 Jul 1;167(1):163-72.
PubMed
Krop I, Shaffer AL, Fearon DT, Schlissel MS: The signaling activity of murine CD19 is regulated during cell development. J Immunol. 1996 Jul 1;157(1):48-56.
PubMed
Variant
0.1 mg
11-596-C100
In stock
80.00 USD
0.025 mg
11-596-C025
Out of stock
40.00 USD
Variant
0.1 mg
11-261-C100
In stock
80.00 USD
0.1 mg
11-261-C100
In stock
40.00 USD
Contact distributor
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SDS download
Bulk requests
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