Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD19 Purified

Anti-Ms CD19 Purified

Regulatory status

RUO

Antigen

CD19

Clone

1D3

Format

Purified

Reactivity

Mouse

Application

IP, FC (QC tested), IHC(F)

Variant

0.1 mg

11-596-C100

In stock

80.00 USD

0.025 mg

11-596-C025

Delivery 1 week

40.00 USD

Variant

0.1 mg

11-596-C100

In stock

80.00 USD

0.025 mg

11-596-C025

Delivery 1 week

40.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Rat IgG2a

Specificity

The rat monoclonal antibody 1D3 detects an extracellular epitope of murine CD19, 95 kDa type I transmembrane glycoprotein (immunoglobulin superfamily) expressed on B lymphocytes and follicular dendritic cells; it is lost on plasma cells.

Application

IP, FC (QC tested), IHC(F)

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Mouse

Immunogen

Mouse CD19-transfected cell line

Concentration

1 mg/ml

Preparation

Purified by protein-G affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

B4, Leu-12, Cvid

Antigen description

CD19 is a transmembrane glycoprotein of Ig superfamily expressed by B cells from the time of heavy chain rearrangement until plasma cell differentiation. It forms a tetrameric complex with CD21 (complement receptor type 2), CD81 (TAPA-1) and Leu13. Together with BCR (B cell antigen receptor), this complex signals to decrease B cell treshold for activation by the antigen. Besides being signal-amplifying coreceptor for BCR, CD19 can also signal independently of BCR coligation and it turns out to be a central regulatory component upon which multiple signaling pathways converge. Mutation of the CD19 gene results in hypogammaglobulinemia, whereas CD19 overexpression causes B cell hyperactivity.

Entrez Gene ID 12478

UniProt ID P25918

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD19 (1D3) purified antibody (concentration in sample 0,6 μg/ml) DAR APC.

Separation of murine CD19 positive splenocytes (red-filled) from CD19 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD19 (1D3) purified antibody (concentration in sample 0,6 μg/ml) DAR APC.

Product specific references:

Shoham T, Rajapaksa R, Boucheix C, Rubinstein E, Poe JC, Tedder TF, Levy S: The tetraspanin CD81 regulates the expression of CD19 during B cell development in a postendoplasmic reticulum compartment. J Immunol. 2003 Oct 15;171(8):4062-72.
PubMed

Inabe K, Kurosaki T: Tyrosine phosphorylation of B-cell adaptor for phosphoinositide 3-kinase is required for Akt activation in response to CD19 engagement. Blood. 2002 Jan 15;99(2):584-9.
PubMed

Krop I, de Fougerolles AR, Hardy RR, Allison M, Schlissel MS, Fearon DT: Self-renewal of B-1 lymphocytes is dependent on CD19. Eur J Immunol. 1996 Jan;26(1):238-42.
PubMed

Cherukuri A, Cheng PC, Pierce SK: The role of the CD19/CD21 complex in B cell processing and presentation of complement-tagged antigens. J Immunol. 2001 Jul 1;167(1):163-72.
PubMed

Krop I, Shaffer AL, Fearon DT, Schlissel MS: The signaling activity of murine CD19 is regulated during cell development. J Immunol. 1996 Jul 1;157(1):48-56.
PubMed

Variant

0.1 mg

11-596-C100

In stock

80.00 USD

0.025 mg

11-596-C025

Delivery 1 week

40.00 USD

Variant

0.1 mg

11-596-C100