Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD18 Purified

Anti-Ms CD18 Purified

Regulatory status

RUO

Antigen

CD18

Clone

M18/2

Format

Purified

Reactivity

Mouse

Application

IP, WB, FC (QC tested), IHC(F), IHC(P)

Variant

0.1 mg

11-623-C100

In stock

88.00 USD

Variant

0.1 mg

11-623-C100

In stock

88.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a

Specificity

The rat monoclonal antibody M18/2 recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.

Application

IP, WB, FC (QC tested), IHC(F), IHC(P)

Application details

Flow cytometry: Recommended dilution: 1 μg/ml.

Reactivity

Mouse

Negative species

Dog

Immunogen

Murine cytotoxic T cell glycoproteins

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Integrin beta2, Complement receptor C3 subunit beta, ITGB2

Antigen description

CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

Entrez Gene ID 16414

UniProt ID P11835

Flow cytometry surface staining pattern of murine splenocytes stained using anti-mouse CD18 (M18/2) purified antibody (concentration in sample 16 μg/ml) DAR APC.

Separation of murine myeloid cells stained using anti-mouse CD18 (M18/2) purified antibody (concentration in sample 16 μg/ml, DAR APC, red-filled) from murine myeloid cells unstained by primary antibody (DAR APC, black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension.

Product specific references:

Cullere X, Lauterbach M, Tsuboi N, Mayadas TN: Neutrophil-selective CD18 silencing using RNA interference in vivo. Blood. 2008 Apr 1;111(7):3591-8.
PubMed

Abraham C, Miller J: Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1. J Immunol. 2001 Nov 1;167(9):5193-201.
PubMed

Barlow SC, Langston W, Matthews KM, Chidlow JH Jr, Kevil CG: CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes. Am J Pathol. 2004 Dec;165(6):1849-52.
PubMed

Varga G, Balkow S, Wild MK, Stadtbaeumer A, Krummen M, Rothoeft T, Higuchi T, Beissert S, Wethmar K, Scharffetter-Kochanek K, Vestweber D, Grabbe S: Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation. Blood. 2007 Jan 15;109(2):661-9.
PubMed

Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983 Aug 1;158(2):586-602.
PubMed

Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18). J Immunol. 1993 May 15;150(10):4466-77.
PubMed

Zahálka MA, Naor D: Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness. Int Immunol. 1994 Jun;6(6):917-24.
PubMed

Watts GM, Beurskens FJ, Martin Padura, Ballantyne CM, Klickstein LB, Brenner MB, Lee DM: Manifestations of inflammatory arthritis are critically dependent on LFA-1. J Immunol. 2005 Mar 15;174(6):3668-75.
PubMed

Avni O, Pur Z, Yefenof E, Baniyash M: Complement receptor 3 of macrophages is associated with galectin-1-like protein. J Immunol. 1998 Jun 15;160(12):6151-8.
PubMed

Sakurai E, Taguchi H, Anand A, Ambati BK, Gragoudas ES, Miller JW, Adamis AP, Ambati J: Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003 Jun;44(6):2743-9.
PubMed