Anti-Ms CD18 FITC

Anti-Ms CD18 FITC
Regulatory status
RUO
Antigen
CD18
Clone
M18/2
Format
FITC
Reactivity
Mouse
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-623-C100
Ask for delivery term HERE
165.00 USD
Variant
0.1 mg
1F-623-C100
Ask for delivery term HERE
165.00 USD
Product details
Description
Images
References
SDS download
Isotype
Rat IgG2a
Specificity
The rat monoclonal antibody M18/2 recognizes an extracellular epitope of CD18 antigen (integrin beta2 subunit; beta2 integrin), a 95 kDa type I transmembrane protein expressed on all leukocytes.
Application
Application details
Flow cytometry: Recommended dilution: 1-2 μg/ml.
Reactivity
Mouse
Negative species
Dog
Immunogen
Murine cytotoxic T cell glycoproteins
Concentration
0.5 mg/ml
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Integrin beta2, Complement receptor C3 subunit beta, ITGB2
Antigen description
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Entrez Gene ID 16414
UniProt ID P11835
1F-623 FC
Flow cytometry analysis (surface staining) of murine splenocytes using anti-mouse CD18 (M18/2) FITC antibody (red, concentration in sample 3 μg/ml) with blank sample (grey).

Product specific references:

Cullere X, Lauterbach M, Tsuboi N, Mayadas TN: Neutrophil-selective CD18 silencing using RNA interference in vivo. Blood. 2008 Apr 1;111(7):3591-8.
PubMed
Abraham C, Miller J: Molecular mechanisms of IL-2 gene regulation following costimulation through LFA-1. J Immunol. 2001 Nov 1;167(9):5193-201.
PubMed
Barlow SC, Langston W, Matthews KM, Chidlow JH Jr, Kevil CG: CD18 deficiency protects against multiple low-dose streptozotocin-induced diabetes. Am J Pathol. 2004 Dec;165(6):1849-52.
PubMed
Varga G, Balkow S, Wild MK, Stadtbaeumer A, Krummen M, Rothoeft T, Higuchi T, Beissert S, Wethmar K, Scharffetter-Kochanek K, Vestweber D, Grabbe S: Active MAC-1 (CD11b/CD18) on DCs inhibits full T-cell activation. Blood. 2007 Jan 15;109(2):661-9.
PubMed
Sanchez-Madrid F, Simon P, Thompson S, Springer TA: Mapping of antigenic and functional epitopes on the alpha- and beta-subunits of two related mouse glycoproteins involved in cell interactions, LFA-1 and Mac-1. J Exp Med. 1983 Aug 1;158(2):586-602.
PubMed
Blocking lymphoma invasiveness with a monoclonal antibody directed against the beta-chain of the leukocyte adhesion molecule (CD18). J Immunol. 1993 May 15;150(10):4466-77.
PubMed
Zahálka MA, Naor D: Beta 2-integrin dependent aggregate formation between LB T cell lymphoma and spleen cells: assessment of correlation with spleen invasiveness. Int Immunol. 1994 Jun;6(6):917-24.
PubMed
Watts GM, Beurskens FJ, Martin Padura, Ballantyne CM, Klickstein LB, Brenner MB, Lee DM: Manifestations of inflammatory arthritis are critically dependent on LFA-1. J Immunol. 2005 Mar 15;174(6):3668-75.
PubMed
Avni O, Pur Z, Yefenof E, Baniyash M: Complement receptor 3 of macrophages is associated with galectin-1-like protein. J Immunol. 1998 Jun 15;160(12):6151-8.
PubMed
Sakurai E, Taguchi H, Anand A, Ambati BK, Gragoudas ES, Miller JW, Adamis AP, Ambati J: Targeted disruption of the CD18 or ICAM-1 gene inhibits choroidal neovascularization. Invest Ophthalmol Vis Sci. 2003 Jun;44(6):2743-9.
PubMed
Variant
0.1 mg
1F-623-C100
Ask for delivery term HERE
165.00 USD
Variant
0.1 mg
1F-623-C100
Ask for delivery term HERE
165.00 USD

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