Anti-Ms CD16/CD32 FITC

Anti-Ms CD16/CD32 FITC
Regulatory status
RUO
Antigen
CD16/CD32
Clone
93
Format
FITC
Reactivity
Mouse
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-141-C100
Ask for delivery term HERE
110.00 USD

0.025 mg
1F-141-C025
Delivery 1 week
55.00 USD
Variant
0.1 mg
1F-141-C100
Ask for delivery term HERE
110.00 USD

0.025 mg
1F-141-C025
Delivery 1 week
55.00 USD
Product details
Description
Images
References
SDS download
Isotype
Rat IgG2a lambda
Specificity
The rat monoclonal antibody 93 recognizes a common extracellular epitope of murine CD16 (FcgammaRIII) and CD32 (FcgammaRII), the low affinity receptors for IgG.
Application
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Mouse
Immunogen
Murine pre-B cells
Concentration
0.5 mg/ml
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CD16, CD32, FcgammaRIII, FCgammaRII, FCGR3, FCGR2
Antigen description
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Unlike human, the murine protein is expressed only as a transmembrane isoform. Also CD32 (FcgammaRII) is a low affinity receptor for IgG, but its affinity is lower than that of CD16. These receptors are expressed on monocytes/macrophages, NK cells, granulocytes, mast cells, dendritic cells, and B cells. Their role is to mediate adaptive immune responses through binding the antibody-antigen immunocomplexes, but their effect on the particular cell differs according to the cell type.
1F-141_FC_Profil Značení
Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD16/32 (93) FITC antibody (concentration in sample 15 μg/ml).
1F-141_FC_Histogram
Separation of murine CD16/32 positive cells (red-filled) from murine CD16/32 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD16/32 (93) FITC antibody (concentration in sample 15 μg/ml).

Product specific references:

Oliver AM, Martin F, Kearney JF: Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. J Immunol. 1997 Feb 1;158(3):1108-15.
PubMed
Welch JS, Yuan W, Ley TJ: PML-RARA can increase hematopoietic self-renewal without causing a myeloproliferative disease in mice. J Clin Invest. 2011 Apr;121(4):1636-45. doi: 10.1172/JCI42953.
PubMed
Somervaille TC, Cleary ML: Identification and characterization of leukemia stem cells in murine MLL-AF9 acute myeloid leukemia. Cancer Cell. 2006 Oct;10(4):257-68.
PubMed
Clémenceau B, Vivien R, Pellat C, Foss M, Thibault G, Vié H: The human natural killer cytotoxic cell line NK-92, once armed with a murine CD16 receptor, represents a convenient cellular tool for the screening of mouse mAbs according to their ADCC potential. MAbs. 2013 Jul-Aug;5(4):587-94. doi: 10.4161/mabs.25077.
PubMed
Siggs OM, Li X, Xia Y, Beutler B: ZBTB1 is a determinant of lymphoid development. J Exp Med. 2012 Jan 16;209(1):19-27.
PubMed
Böiers C, Buza-Vidas N, Jensen CT, Pronk CJ, Kharazi S, Wittmann L, Sitnicka E, Hultquist A, Jacobsen SE: Expression and role of FLT3 in regulation of the earliest stage of normal granulocyte-monocyte progenitor development. Blood. 2010 Jun 17;115(24):5061-8. doi: 10.1182/blood-2009-12-258756.
PubMed
Variant
0.1 mg
1F-141-C100
Ask for delivery term HERE
110.00 USD

0.025 mg
1F-141-C025
Delivery 1 week
55.00 USD
Variant
0.1 mg
1F-141-C100
Ask for delivery term HERE
110.00 USD

0.025 mg
1F-141-C025
Delivery 1 week
55.00 USD

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