Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD16/CD32 FITC

Anti-Ms CD16/CD32 FITC

Regulatory status

RUO

Antigen

CD16/CD32

Clone

Format

FITC

Reactivity

Mouse

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1F-141-C100

Ask for delivery term HERE

110.00 USD

0.025 mg

1F-141-C025

Delivery 1 week

55.00 USD

Variant

0.1 mg

1F-141-C100

Ask for delivery term HERE

110.00 USD

0.025 mg

1F-141-C025

Delivery 1 week

55.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Rat IgG2a lambda

Specificity

The rat monoclonal antibody 93 recognizes a common extracellular epitope of murine CD16 (FcgammaRIII) and CD32 (FcgammaRII), the low affinity receptors for IgG.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 2-5 μg/ml.

Reactivity

Mouse

Immunogen

Murine pre-B cells

Concentration

0.5 mg/ml

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CD16, CD32, FcgammaRIII, FCgammaRII, FCGR3, FCGR2

Antigen description

CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Unlike human, the murine protein is expressed only as a transmembrane isoform. Also CD32 (FcgammaRII) is a low affinity receptor for IgG, but its affinity is lower than that of CD16. These receptors are expressed on monocytes/macrophages, NK cells, granulocytes, mast cells, dendritic cells, and B cells. Their role is to mediate adaptive immune responses through binding the antibody-antigen immunocomplexes, but their effect on the particular cell differs according to the cell type.

Flow cytometry surface staining pattern of murine splenocyte suspension stained using anti-mouse CD16/32 (93) FITC antibody (concentration in sample 15 μg/ml).

Separation of murine CD16/32 positive cells (red-filled) from murine CD16/32 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-mouse CD16/32 (93) FITC antibody (concentration in sample 15 μg/ml).

Product specific references:

Oliver AM, Martin F, Kearney JF: Mouse CD38 is down-regulated on germinal center B cells and mature plasma cells. J Immunol. 1997 Feb 1;158(3):1108-15.
PubMed

Welch JS, Yuan W, Ley TJ: PML-RARA can increase hematopoietic self-renewal without causing a myeloproliferative disease in mice. J Clin Invest. 2011 Apr;121(4):1636-45. doi: 10.1172/JCI42953.
PubMed

Somervaille TC, Cleary ML: Identification and characterization of leukemia stem cells in murine MLL-AF9 acute myeloid leukemia. Cancer Cell. 2006 Oct;10(4):257-68.
PubMed

Clémenceau B, Vivien R, Pellat C, Foss M, Thibault G, Vié H: The human natural killer cytotoxic cell line NK-92, once armed with a murine CD16 receptor, represents a convenient cellular tool for the screening of mouse mAbs according to their ADCC potential. MAbs. 2013 Jul-Aug;5(4):587-94. doi: 10.4161/mabs.25077.
PubMed

Siggs OM, Li X, Xia Y, Beutler B: ZBTB1 is a determinant of lymphoid development. J Exp Med. 2012 Jan 16;209(1):19-27.
PubMed

Böiers C, Buza-Vidas N, Jensen CT, Pronk CJ, Kharazi S, Wittmann L, Sitnicka E, Hultquist A, Jacobsen SE: Expression and role of FLT3 in regulation of the earliest stage of normal granulocyte-monocyte progenitor development. Blood. 2010 Jun 17;115(24):5061-8. doi: 10.1182/blood-2009-12-258756.
PubMed