Anti-Ms CD154 Purified Low Endotoxin

Anti-Ms CD154 Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD154
Clone
MR-1
Format
Purified Low Endotoxin
Reactivity
Mouse
Variant
0.1 mg
12-629-C100
In stock
132.00 USD
Variant
0.1 mg
12-629-C100
In stock
132.00 USD
Product details
Description
Images
References
SDS download
Isotype
Armenian Hamster IgG1
Specificity
The Armenian hamster monoclonal antibody MR-1 detects an extracellular epitope on murine CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.
Application details
Functional application:
Blocking T cell costimulation in vivo.
Flow cytometry: Recommended dilution: 4 μg/ml; positive control: 6-8 hour activated mouse splenocytes.
Reactivity
Mouse
Immunogen
Activated mouse Th1 clone D1.6
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM
Antigen description
CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.
Entrez Gene ID 959
UniProt ID P29965
12-629_FC_Profil Značení
Flow cytometry surface staining pattern of murine PMA, ionomycin and LPS stimulated splenocytes stained using anti-mouse CD154 (MR-1) purified antibody (low endotoxin, concentration in sample 3 μg/ml, GAArH APC).
12-629_FC_Histogram
Separation of CD154 positive cells (red-filled) from CD154 negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine PMA, ionomycin and LPS stimulated splenocytes stained using anti-mouse CD154 (MR-1) purified antibody (low endotoxin, concentration in sample 3 μg/ml, GAArH APC).

Product specific references:

Lode HN, Xiang R, Pertl U, Förster E, Schoenberger SP, Gillies SD, Reisfeld RA: Melanoma immunotherapy by targeted IL-2 depends on CD4(+) T-cell help mediated by CD40/CD40L interaction. J Clin Invest. 2000 Jun;105(11):1623-30.
PubMed
Fischbein MP, Ardehali A, Yun J, Schoenberger S, Laks H, Irie Y, Dempsey P, Cheng G, Fishbein MC, Bonavida B: CD40 signaling replaces CD4+ lymphocytes and its blocking prevents chronic rejection of heart transplants. J Immunol. 2000 Dec 15;165(12):7316-22.
PubMed
Hwang Y, Nahm MH, Briles DE, Thomas D, Purkerson JM: Acquired, but not innate, immune responses to Streptococcus pneumoniae are compromised by neutralization of CD40L. Infect Immun. 2000 Feb;68(2):511-7.
PubMed
Murakawa T, Kerklo MM, Zamora MR, Wei Y, Gill RG, Henson PM, Grover FL, Nicolls MR: Simultaneous LFA-1 and CD40 ligand antagonism prevents airway remodeling in orthotopic airway transplantation: implications for the role of respiratory epithelium as a modulator of fibrosis. J Immunol. 2005 Apr 1;174(7):3869-79.
PubMed
Salgado CG, Nakamura K, Sugaya M, Tada Y, Asahina A, Koyama Y, Irie S, Tamaki K: Functional CD40 ligand is expressed on epidermal Langerhans cells. J Leukoc Biol. 1999 Aug;66(2):281-5.
PubMed
Wu Y, Zhou Q, Zheng P, Liu Y: CD28-independent induction of T helper cells and immunoglobulin class switches requires costimulation by the heat-stable antigen. J Exp Med. 1998 Apr 6;187(7):1151-6.
PubMed
Luzina IG, Atamas SP, Storrer CE, daSilva LC, Kelsoe G, Papadimitriou JC, Handwerger BS: Spontaneous formation of germinal centers in autoimmune mice. J Leukoc Biol. 2001 Oct;70(4):578-84.
PubMed
Shin T, Kennedy G, Gorski K, Tsuchiya H, Koseki H, Azuma M, Yagita H, Chen L, Powell J, Pardoll D, Housseau F: Cooperative B7-1/2 (CD80/CD86) and B7-DC costimulation of CD4+ T cells independent of the PD-1 receptor. J Exp Med. 2003 Jul 7;198(1):31-8.
PubMed
Howard LM, Miga AJ, Vanderlugt CL, Dal Canto MC, Laman JD, Noelle RJ, Miller SD: Mechanisms of immunotherapeutic intervention by anti-CD40L (CD154) antibody in an animal model of multiple sclerosis. J Clin Invest. 1999 Jan;103(2):281-90.
PubMed
Noelle RJ, Roy M, Shepherd DM, Stamenkovic I, Ledbetter JA, Aruffo A: A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc Natl Acad Sci U S A. 1992 Jul 15;89(14):6550-4.
PubMed
Variant
0.1 mg
12-629-C100
In stock
132.00 USD
Variant
0.1 mg
12-629-C100
In stock
132.00 USD

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