Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms CD11a Purified Low Endotoxin

Anti-Ms CD11a Purified Low Endotoxin

Anti-Ms CD11a Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD11a
Clone
M17/4
Format
Purified Low Endotoxin
Reactivity
Mouse
Application
IP, FC (QC tested), IHC(F), FUNC
Variant
0.1 mg
12-622-C100
In stock
120.00 USD
Variant
0.1 mg
11-261-C100
In stock
120.00 USD
Contact distributor
Product details
Images
References
Isotype
Rat IgG2a
Specificity
The rat monoclonal antibody M17/4 reacts with an extracellular epitope of CD11a (alpha-subunit of murine LFA-1), a 180 kDa type I transmembrane glycoprotein expressed on B and T lymphocytes, monocytes, macrophages, neutrophils, basophils and eosinophils.
Application
IP, FC (QC tested), IHC(F), FUNC
Application details
Functional application: Blocking.
Flow cytometry: Recommended dilution: 1 μg/ml.
Immunohistochemistry (frozen sections): Positive tissue: murine spleen or thymus, acetone fixation.
Reactivity
Mouse
Immunogen
C57BL/6 mouse splenic secondary cytotoxic T lymphocytes
Other names
LFA-1, LFA1A, ITGAL
Concentration
1 mg/ml
Preparation
Purified by protein-G affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
12-622_FC_Profil Značení
Flow cytometry surface staining pattern of murine splenocytes stained using anti-mouse CD11a (M17/4) purified antibody (low endotoxin, concentration in sample 0,6 μg/ml) DAR APC.
12-622_FC_Histogram
Separation of murine myeloid cells (red-filled) from cellular debris (black-dashed) in flow cytometry analysis (surface staining) of murine splenocytes stained using anti-mouse CD11a (M17/4) purified antibody (low endotoxin, concentration in sample 0,6 μg/ml) DAR APC.

Product specific references:

Sanchez-Madrid F, Davignon D, Martz E, Springer TA: Antigens involved in mouse cytolytic T-lymphocyte (CTL)-mediated killing: functional screening and topographic relationship. Cell Immunol. 1982 Oct;73(1):1-11.
PubMed
Kuhlman P, Moy VT, Lollo BA, Brian AA: The accessory function of murine intercellular adhesion molecule-1 in T lymphocyte activation. Contributions of adhesion and co-activation. J Immunol. 1991 Mar 15;146(6):1773-82.
PubMed
Angelov GS, Guillaume P, Cebecauer M, Bosshard G, Dojcinovic D, Baumgaertner P, Luescher IF: Soluble MHC-peptide complexes containing long rigid linkers abolish CTL-mediated cytotoxicity. J Immunol. 2006 Mar 15;176(6):3356-65.
PubMed
Smith SS, Barnum SR: Differential expression of beta 2-integrins and cytokine production between gammadelta and alphabeta T cells in experimental autoimmune encephalomyelitis. J Leukoc Biol. 2008 Jan;83(1):71-9.
PubMed
Yung R, Powers D, Johnson K, Amento E, Carr D, Laing T, Yang J, Chang S, Hemati N, Richardson B: Mechanisms of drug-induced lupus. II. T cells overexpressing lymphocyte function-associated antigen 1 become autoreactive and cause a lupuslike disease in syngeneic mice. J Clin Invest. 1996 Jun 15;97(12):2866-71.
PubMed
Bellingan GJ, Xu P, Cooksley H, Cauldwell H, Shock A, Bottoms S, Haslett C, Mutsaers SE, Laurent GJ: Adhesion molecule-dependent mechanisms regulate the rate of macrophage clearance during the resolution of peritoneal inflammation. J Exp Med. 2002 Dec 2;196(11):1515-21.
PubMed
Beyer M, Wang H, Peters N, Doths S, Koerner-Rettberg C, Openshaw PJ, Schwarze J: The beta2 integrin CD11c distinguishes a subset of cytotoxic pulmonary T cells with potent antiviral effects in vitro and in vivo. Respir Res. 2005 Jul 12;6:70.
PubMed
Koseki S, Miura S, Fujimori H, Hokari R, Komoto S, Hara Y, Ogino T, Nagata H, Goto M, Hachimura S, Kaminogawa S, Ishii H: In situ demonstration of intraepithelial lymphocyte adhesion to villus microvessels of the small intestine. Int Immunol. 2001 Sep;13(9):1165-74.
PubMed
Papayannopoulou T, Priestley GV, Nakamoto B, Zafiropoulos V, Scott LM, Harlan JM: Synergistic mobilization of hemopoietic progenitor cells using concurrent beta1 and beta2 integrin blockade or beta2-deficient mice. Blood. 2001 Mar 1;97(5):1282-8.
PubMed
Variant
0.1 mg
12-622-C100
In stock
120.00 USD
Variant
0.1 mg
11-261-C100
In stock
120.00 USD
Contact distributor
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