Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu TROP2 Purified

Anti-Hu TROP2 Purified

Regulatory status

RUO

Antigen

TROP2

Clone

TrMab-6

Format

Purified

Reactivity

Pig, Human

Application

IHC, WB, FC (QC tested), ICC

Variant

0.1 mg

11-898-C100

In stock

158.40 USD

Variant

0.1 mg

11-898-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody TrMab-6 recognizes an extracellular epitope of TROP2, a type I transmembrane glycoprotein. This antibody is usefull for detection of TROP2 in breast cancer.

Application

IHC, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.
Western blotting: Recommended dilution: 1 μg/ml.
Immunocytochemistry: Recommended dilution: 10 μg/ml; the cells can be fixed with 4% PFA and permeabilized with 0.1% Triton X-100 before antibody staining, when needed.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml. Heat-mediated antigen retrieval in citrate buffer, pH = 6.

Reactivity

Pig, Human

Negative species

Mouse

Immunogen

TROP2-transfected CHO cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TACSTD2, EGP1, GP50, M1S1, GA733-1

Antigen description

TROP2 is a cell surface receptor that transduces calcium signals. It belongs to carcinoma-associated antigens. Mutations of TROP2 have been associated with gelatinous drop-like corneal dystrophy.

Entrez Gene ID 4070

UniProt ID P09758

Western blotting analysis of human TROP2 using mouse monoclonal antibody TrMab-6 on lysates of MCF-7, SK-BR-3, and HaCaT cell lines, TROP2-positive and TROP2-negative DU-145 cells, wild-type T-47D and TROP2 knock-out T-47D cells, and TROP2 over-expressing and knock-out MDA-MB-231 cells. Primary antibody concentration was 1 µg/ml. Tested by Ondřej Vacek and Karel Souček, Institute of Biophysics of the Czech Academy of Sciences.

Separation of A431 cells (red-filled) from human peripheral whole blood cells (black-dashed) in flow cytometry analysis (surface staining) stained using anti-human TROP-2 (TrMab-6) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Immunocytochemistry staining of TROP2 on the surface of unfixed wild-type and TROP2-overexpressing MDA-MB-231 cells using mouse monoclonal antibody TrMab-6 (1 µg/ml), detected with GAM IgG-Alexa Fluor®488 (diluted 1:1000, green); isotype control IgG2b was used as the negative control. DNA stained with DAPI (blue). Tested by Ondřej Vacek and Karel Souček, Institute of Biophysics of the Czech Academy of Sciences.

Immunohistochemistry staining (paraffin sections) of TROP2 in human and porcine lung tissue using mouse monoclonal antibody TrMab-6 (diluted 1:100), detected with En Vision FLEX/HRP. Positive in basolateral parts of airway epithelium. Negative control was stained without primary antibody- Scale bar represents 100 µm. Tested by Vendula Pelková and Aleš Hampl, Department of Histology and Embryology at Faculty of Medicine, Masaryk University, Czech Republic.

Product specific references:

Sayama Y, Kaneko MK, Kato Y: Development and characterization of TrMab‑6, a novel anti‑TROP2 monoclonal antibody for antigen detection in breast cancer. Mol Med Rep. 2021 Feb;23(2):92.
PubMed