Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu TCR Vgamma9 PE-Cy™7

Anti-Hu TCR Vgamma9 PE-Cy™7

Regulatory status

RUO

Antigen

TCR Vgamma9

Clone

Format

PE-Cy™7

Reactivity

Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T7-903-T100

In stock

282.70 USD

Variant

100 tests

T7-903-T100

In stock

282.70 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody B3 recognizes an extracellular epitope of human TCR Vgamma9, a variant of TCR gamma chain, which is expressed on a gamma/delta T cell subpopulation with cytolytic activity against various tumor cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human, Non-human primates

Immunogen

human T cells

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Cy™ and CyDye™ are registered trademarks of Cytiva.

Other names

TCRVG9

Antigen description

TCR Vgamma9 is a variant of TCR gamma chain, that is present on a subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Vgamma9 (B3) PE-Cy™7 antibody (concentration in sample 1.67 μg/ml).

Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human TCR Vgamma9 (B3) PE-Cy™7 antibody (concentration in sample 1.67 μg/ml) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human TCR Vgamma9 positive CD3 positive T cells (red-filled) from TCR Vgamma9 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Vgamma9 (B3) PE-Cy™7 antibody (concentration in sample 1.67 μg/ml).

Product specific references:

van Rhijn I, van den Berg LH, Ang CW, Admiraal J, Logtenberg T: Expansion of human gammadelta T cells after in vitro stimulation with Campylobacter jejuni. Int Immunol. 2003 Mar;15(3):373-82.
PubMed