Anti-Hu TCR Vgamma9 PE

Anti-Hu TCR Vgamma9 PE
Regulatory status
RUO
Antigen
TCR Vgamma9
Clone
B3
Format
PE
Reactivity
Human, Non-human primates
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-903-T100
In stock
242.00 USD
Variant
100 tests
1P-903-T100
In stock
242.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody B3 recognizes an extracellular epitope of human TCR Vgamma9, a variant of TCR gamma chain, which is expressed on a gamma/delta T cell subpopulation with cytolytic activity against various tumor cells.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human, Non-human primates
Immunogen
human T cells
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TCRVG9
Antigen description
TCR Vgamma9 is a variant of TCR gamma chain, that is present on a subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors.
1P-903_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Vgamma9 (B3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-903_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR Vgamma9 (B3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (5 μl reagent / 100 μl of peripheral whole blood).
1P-903_FC_Histogram
Separation of human TCR Vgamma9 positive lymphocytes (red-filled) from human TCR Vgamma9 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR Vgamma9 (B3) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

van Rhijn I, van den Berg LH, Ang CW, Admiraal J, Logtenberg T: Expansion of human gammadelta T cells after in vitro stimulation with Campylobacter jejuni. Int Immunol. 2003 Mar;15(3):373-82.
PubMed
Variant
100 tests
1P-903-T100
In stock
242.00 USD
Variant
100 tests
1P-903-T100
In stock
242.00 USD

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