Anti-Hu TCR Vdelta2 Purified

Anti-Hu TCR Vdelta2 Purified
Regulatory status
RUO
Antigen
TCR Vdelta2
Clone
B6
Format
Purified
Reactivity
Human
Application
FC (QC tested)
Variant
0.1 mg
11-905-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody B6 recognizes an extracellular epitope of human TCR Vdelta2, which is expressed on the majority of gamma/delta T cells.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml.
Reactivity
Human
Immunogen
human recombinant TCR Vdelta2
Other names
TCRVD2
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-905_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Vdelta2 (B6) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).
11-905_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human lymphocytes using anti-human TCR Vdelta2 (B6) purified antibody (concentration in sample 0,3 μg/ml, GAM APC) and anti-human CD3 (UCHT1) PE antibody (4 μl reagent / 100 μl of peripheral whole blood).
11-905_FC_Histogram
Separation of human TCR Vdelta2 positive lymphocytes (red-filled) from human TCR Vdelta2 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR Vdelta2 (B6) purified antibody (concentration in sample 0,3 μg/ml, GAM APC).

Product specific references:

Siegers GM, Felizardo TC, Mathieson AM, Kosaka Y, Wang XH, Medin JA, Keating A: Anti-leukemia activity of in vitro-expanded human gamma delta T cells in a xenogeneic Ph+ leukemia model. PLoS One. 2011 Feb 3;6(2):e16700.
PubMed
Hunter S, Willcox CR, Davey MS, Kasatskaya SA, Jeffery HC, Chudakov DM, Oo YH, Willcox BE: Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations. J Hepatol. 2018 Sep;69(3):654-665.
PubMed
Ogongo P, Steyn AJ, Karim F, Kaylesh J Dullabh KJ, Awala I, Madansein R, Leslie A, Behar SM: Differential skewing of donor-unrestricted and γδ T cell repertoires in tuberculosis-infected human lungs. J Clin Invest. 2020 Jan 2;130(1):214-230.
PubMed
Li H, Luo K, Pauza CD: TNF-alpha is a positive regulatory factor for human Vgamma2 Vdelta2 T cells. J Immunol. 2008 Nov 15;181(10):7131-7
PubMed
Fisher JP, Yan M, Heuijerjans J, Carter L, Abolhassani A, Frosch J, Wallace R, Flutter B, Capsomidis A, Hubank M, Klein N, Callard R, Gustafsson K, Anderson J: Neuroblastoma killing properties of Vδ2 and Vδ2-negative γδT cells following expansion by artificial antigen-presenting cells. Clin Cancer Res. 2014 Nov 15;20(22):5720-32.
PubMed
Davey MS, Willcox CR, Hunter S, KasatskayaSA, Remmerswaal EB, Salim M, Mohammed F, Bemelman FJ, Chudakov DM, Oo YH, Willcox BE: The human Vδ2 + T-cell compartment comprises distinct innate-like Vγ9 + and adaptive Vγ9 - subsets. Nat Commun. 2018 May 2;9(1):1760.
PubMed
Variant
0.1 mg
11-905-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD