Anti-Hu TCR Vdelta2 PE

Anti-Hu TCR Vdelta2 PE
Regulatory status
RUO
Antigen
TCR Vdelta2
Clone
B6
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1P-905-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody B6 recognizes an extracellular epitope of human TCR Vdelta2, which is expressed on the majority of gamma/delta T cells.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
human recombinant TCR Vdelta2
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TCRVD2
Antigen description
TCR Vdelta2 is a variant of TCR delta chain, that is present on a major subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 (or Vgamma2/Vdelta2) T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors. They can recognize these antigens in an MHC-unrestricted manner. Similarly to NK cells, Vdelta2 T cells express MHC I receptors and killer Ig-like receptors, that are involved in tumor recognition and cytolysis. The potently cytotoxic subset of them is identified by cell surface expression of polysialyated CD56.
1P-905_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Vdelta2 (B6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-905_FC_Dot-Plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR Vdelta2 (B6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1P-905_FC_Histogram
Separation of human TCR Vdelta2 positive lymphocytes (red-filled) from TCR Vdelta2 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Vdelta2 (B6) PE antibody (10 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Siegers GM, Felizardo TC, Mathieson AM, Kosaka Y, Wang XH, Medin JA, Keating A: Anti-leukemia activity of in vitro-expanded human gamma delta T cells in a xenogeneic Ph+ leukemia model. PLoS One. 2011 Feb 3;6(2):e16700.
PubMed
Hunter S, Willcox CR, Davey MS, Kasatskaya SA, Jeffery HC, Chudakov DM, Oo YH, Willcox BE: Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations. J Hepatol. 2018 Sep;69(3):654-665.
PubMed
Ogongo P, Steyn AJ, Karim F, Kaylesh J Dullabh KJ, Awala I, Madansein R, Leslie A, Behar SM: Differential skewing of donor-unrestricted and γδ T cell repertoires in tuberculosis-infected human lungs. J Clin Invest. 2020 Jan 2;130(1):214-230.
PubMed
Li H, Luo K, Pauza CD: TNF-alpha is a positive regulatory factor for human Vgamma2 Vdelta2 T cells. J Immunol. 2008 Nov 15;181(10):7131-7
PubMed
Fisher JP, Yan M, Heuijerjans J, Carter L, Abolhassani A, Frosch J, Wallace R, Flutter B, Capsomidis A, Hubank M, Klein N, Callard R, Gustafsson K, Anderson J: Neuroblastoma killing properties of Vδ2 and Vδ2-negative γδT cells following expansion by artificial antigen-presenting cells. Clin Cancer Res. 2014 Nov 15;20(22):5720-32.
PubMed
Davey MS, Willcox CR, Hunter S, KasatskayaSA, Remmerswaal EB, Salim M, Mohammed F, Bemelman FJ, Chudakov DM, Oo YH, Willcox BE: The human Vδ2 + T-cell compartment comprises distinct innate-like Vγ9 + and adaptive Vγ9 - subsets. Nat Commun. 2018 May 2;9(1):1760.
PubMed
Variant
100 tests
1P-905-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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