Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu TCR Valpha24-Jalpha18 Purified

Anti-Hu TCR Valpha24-Jalpha18 Purified

Regulatory status

RUO

Antigen

TCR Valpha24-Jalpha18

Clone

6B11

Format

Purified

Reactivity

Human, Non-human primates

Application

FC (QC tested), IHC-F, ICC

Variant

0.1 mg

11-927-C100

In stock

158.40 USD

Variant

0.1 mg

11-927-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 6B11 recognizes an extracellular epitope within V alpha 24 J alpha 18 TCR, which is expressed by invariant NK T cells (iNKT).

Application

FC (QC tested), IHC-F, ICC

Application details

Flow cytometry: Recommended dilution: 1-5 µg/ml

Reactivity

Human, Non-human primates

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

TCR alpha V24-J18 chain is a marker of invariant NK T cells (iNKT). This chain pairs mostly with TCR beta V11 chain, and together they interact with glycolipide-loaded CD1d. This interaction results in iNKT activation and cytokine production (IFN gamma, IL-4, IL-13), which takes important part in immune regulation and host response to pathogens and cancer(support of both Th1 and Th2 way), although iNKT cells represent less than 1% of total peripheral T cells.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Valpha24-Jalpha18 (6B11) purified antibody (concentration in sample 0.56 μg/ml, GAM APC).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR Valpha24-Jalpha18 (6B11) purified antibody (concentration in sample 0.56 μg/ml, GAM APC) and anti-human CD3 (UCHT1) APC antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD3 positive TCR Valpha24-Jalpha18 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Valpha24-Jalpha18 (6B11) purified antibody (concentration in sample 0.56 μg/ml, GAM APC).

Product specific references:

Montoya CJ, Pollard D, Martinson J, Kumari K, Wasserfall C, Mulder CB, Rugeles MT, Atkinson MA, Landay AL, Wilson SB: Characterization of human invariant natural killer T subsets in health and disease using a novel invariant natural killer T cell-clonotypic monoclonal antibody, 6B11. Immunology. 2007 Sep;122(1):1-14.
PubMed

Vyth-Dreese FA, Sein J, van de Kasteele W, Dellemijn TA, van den Bogaard C, Nooijen WJ, de Gast GC, Haanen JB, Bex A: Lack of anti-tumour reactivity despite enhanced numbers of circulating natural killer T cells in two patients with metastatic renal cell carcinoma. Clin Exp Immunol. 2010 Dec;162(3):447-59.
PubMed

Krovi SH, Gapin L: Invariant natural killer T cell subsets - more than just developmental intermediates. Front Immunol. 2018 Jun 20;9:1393.
PubMed

Chamoto K, Guo T, Scally SW, Kagoya Y, Anczurowski M, Wang CH, Rahman MA, Saso K, Butler MO, Chiu PP, Julien JP, Hirano N: Key residues at third CDR3β position impact structure and antigen recognition of human invariant NK TCRs. J Immunol. 2017 Feb 1;198(3):1056-1065.
PubMed