Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu TCR gamma/delta Purified

Anti-Hu TCR gamma/delta Purified

Regulatory status

RUO

Antigen

TCR gamma/delta

Clone

11F2

Format

Purified

Reactivity

Human

Application

ELISA, IP, FC (QC tested), IHC-F

Variant

0.1 mg

11-912-C100

In stock

158.40 USD

Variant

0.1 mg

11-912-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody 11F2 recognizes an extracellular epitope in all molecular forms of the TCR gamma/delta.

Application

ELISA, IP, FC (QC tested), IHC-F

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Human

Immunogen

Purified CD3 antigen complex

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TCRG/D

Antigen description

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Separation of TCR gamma/delta positive lymphocytes (red-filled) from human TCR gamma/delta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human TCR gamma/delta (11F2) purified antibody (concentration in sample 1,7 μg/ml, GAM APC).

Product specific references:

Ichinohasama R, Miura I, Takahashi T, Yaginuma Y, Myers J, DeCoteau JF, Yee C, Kadin ME, Mori S, Sawai T: Peripheral CD4+ CD8- gammadelta T cell lymphoma: a case report with multiparameter analyses. Hum Pathol. 1996 Dec;27(12):1370-7.
PubMed

Futagbi G, Welbeck JE, Tetteh JK, Hviid L, Akanmori BD: Selective activation of TCR-gammadelta+ cells in endemic Burkitt's lymphoma. Malar J. 2007 May 23;6:69.
PubMed

Bertaina A, Zorzoli A, Petretto A, Barbarito G, Inglese E, Merli P, Lavarello C, Brescia LP, De Angelis B, Tripodi G, Moretta L, Locatelli F, Airoldi I: Zoledronic acid boosts γδ T-cell activity in children receiving αβ + T and CD19 + cell-depleted grafts from an HLA-haplo-identical donor. Oncoimmunology. 2016 Sep 27;6(2):e1216291.
PubMed

Papadopoulou M, Tieppo P, McGovern N, Gosselin F, Chan JK, Goetgeluk G, Dauby N, Cogan A, Donner C, Ginhoux F, Vandekerckhove B, Vermijlen D: TCR sequencing reveals the distinct development of fetal and adult human Vγ9Vδ2 T cells. J Immunol. 2019 Sep 15;203(6):1468-1479.
PubMed

Borst J, van Dongen JJ, Bolhuis RL, Peters PJ, Hafler DA, de Vries E, van de Griend RJ: Distinct molecular forms of human T cell receptor gamma/delta detected on viable T cells by a monoclonal antibody. J Exp Med. 1988 May 1;167(5):1625-44.
PubMed