Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu TCR gamma/delta PE-DyLight<sup>®</sup> 594

Anti-Hu TCR gamma/delta PE-DyLight^® 594

Regulatory status

RUO

Antigen

TCR gamma/delta

Clone

Format

PE-DyLight^® 594

Reactivity

Human, Non-human primates

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

T5-147-T100

In stock

275.00 USD

Variant

100 tests

T5-147-T100

In stock

275.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody B1 (also known as B1.1) recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Reactivity

Human, Non-human primates

Preparation

Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-DyLight®594 (PE-DyLight®594) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

DyLight^® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Other names

TCRG/D

Antigen description

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (B1) PE-DyLight® 594 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR gamma/delta (B1) PE-DyLight® 594 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).

Separation of human TCR gamma/delta positive T cells (red-filled) from TCR gamma/delta negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR gamma/delta (B1) PE-DyLight® 594 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Pöllinger B, Junt T, Metzler B, Walker UA, Tyndall A, Allard C, Bay S, Keller R, Raulf F, Di Padova F, O'Reilly T, Horwood NJ, Patel DD, Littlewood-Evans A: Th17 cells, not IL-17+ γδ T cells, drive arthritic bone destruction in mice and humans.
PubMed

Bordignon M, Belloni Fortina A, Pigozzi B, Alaibac M: γδ T cells as potential contributors to the progression of parapsoriasis to mycosis fungoides. Mol Med Rep. 2008 Jul-Aug;1(4):485-8.
PubMed

Correia DV, Fogli M, Hudspeth K, da Silva MG, Mavilio D, Silva-Santos B: Differentiation of human peripheral blood Vδ1+ T cells expressing the natural cytotoxicity receptor NKp30 for recognition of lymphoid leukemia cells. Blood. 2011 Jul 28;118(4):992-1001.
PubMed

Lehmann FS, Terracciano L, Carena I, Baeriswyl C, Drewe J, Tornillo L, De Libero G, Beglinger C: In situ correlation of cytokine secretion and apoptosis in Helicobacter pylori-associated gastritis. Am J Physiol Gastrointest Liver Physiol. 2002 Aug;283(2):G481-8.
PubMed

Rodríguez-Gago M, de Heredia A, Ramírez P, Parrilla P, Aparicio P, Yélamos J: Human anti-porcine gammadelta T-cell xenoreactivity is inhibited by human FasL expression on porcine endothelial cells. Transplantation. 2001 Aug 15;72(3):503-9.
PubMed