Anti-Hu TCR gamma/delta PE-Cy™7

Anti-Hu TCR gamma/delta PE-Cy™7
Regulatory status
RUO
Antigen
TCR gamma/delta
Clone
11F2
Format
PE-Cy™7
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T7-912-T100
In stock
250.00 USD
Variant
0.1 mg
11-261-C100
In stock
250.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody 11F2 recognizes an extracellular epitope in all molecular forms of the TCR gamma/delta.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Purified CD3 antigen complex
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
TCRG/D
Antigen description
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
T7-912_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).
T7-912_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR gamma/delta (11F2) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
T7-912_FC_Histogram
Separation of human TCR gamma/delta positive T cells (red-filled) from TCR gamma/delta negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR gamma/delta (11F2) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Ichinohasama R, Miura I, Takahashi T, Yaginuma Y, Myers J, DeCoteau JF, Yee C, Kadin ME, Mori S, Sawai T: Peripheral CD4+ CD8- gammadelta T cell lymphoma: a case report with multiparameter analyses. Hum Pathol. 1996 Dec;27(12):1370-7.
PubMed
Futagbi G, Welbeck JE, Tetteh JK, Hviid L, Akanmori BD: Selective activation of TCR-gammadelta+ cells in endemic Burkitt's lymphoma. Malar J. 2007 May 23;6:69.
PubMed
Bertaina A, Zorzoli A, Petretto A, Barbarito G, Inglese E, Merli P, Lavarello C, Brescia LP, De Angelis B, Tripodi G, Moretta L, Locatelli F, Airoldi I: Zoledronic acid boosts γδ T-cell activity in children receiving αβ + T and CD19 + cell-depleted grafts from an HLA-haplo-identical donor. Oncoimmunology. 2016 Sep 27;6(2):e1216291.
PubMed
Papadopoulou M, Tieppo P, McGovern N, Gosselin F, Chan JK, Goetgeluk G, Dauby N, Cogan A, Donner C, Ginhoux F, Vandekerckhove B, Vermijlen D: TCR sequencing reveals the distinct development of fetal and adult human Vγ9Vδ2 T cells. J Immunol. 2019 Sep 15;203(6):1468-1479.
PubMed
Variant
100 tests
T7-912-T100
In stock
250.00 USD
Variant
0.1 mg
11-261-C100
In stock
250.00 USD

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