Anti-Hu TCR gamma/delta PE-Cy™7

Anti-Hu TCR gamma/delta PE-Cy™7
Regulatory status
RUO
Antigen
TCR gamma/delta
Clone
B1
Format
PE-Cy™7
Reactivity
Human, Non-human primates
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T7-147-T100
In stock
275.00 USD

25 tests
T7-147-T025
Delivery 1 week
137.50 USD
Variant
100 tests
T7-147-T100
In stock
275.00 USD

25 tests
T7-147-T025
Delivery 1 week
137.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody B1 (also known as B1.1) recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human, Non-human primates
Preparation
Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
TCRG/D
Antigen description
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
T7-147_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR gamma/delta (B1) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).
T7-147_FC_Dot-plot
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-human TCR gamma/delta (B1) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).
T7-147_FC_Histogram
Separation of human CD3 positive TCR gamma/delta positive lymphocytes (red-filled) from CD3 negative TCR gamma/delta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR gamma/delta (B1) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Pöllinger B, Junt T, Metzler B, Walker UA, Tyndall A, Allard C, Bay S, Keller R, Raulf F, Di Padova F, O'Reilly T, Horwood NJ, Patel DD, Littlewood-Evans A: Th17 cells, not IL-17+ γδ T cells, drive arthritic bone destruction in mice and humans.
PubMed
Bordignon M, Belloni Fortina A, Pigozzi B, Alaibac M: γδ T cells as potential contributors to the progression of parapsoriasis to mycosis fungoides. Mol Med Rep. 2008 Jul-Aug;1(4):485-8.
PubMed
Correia DV, Fogli M, Hudspeth K, da Silva MG, Mavilio D, Silva-Santos B: Differentiation of human peripheral blood Vδ1+ T cells expressing the natural cytotoxicity receptor NKp30 for recognition of lymphoid leukemia cells. Blood. 2011 Jul 28;118(4):992-1001.
PubMed
Lehmann FS, Terracciano L, Carena I, Baeriswyl C, Drewe J, Tornillo L, De Libero G, Beglinger C: In situ correlation of cytokine secretion and apoptosis in Helicobacter pylori-associated gastritis. Am J Physiol Gastrointest Liver Physiol. 2002 Aug;283(2):G481-8.
PubMed
Rodríguez-Gago M, de Heredia A, Ramírez P, Parrilla P, Aparicio P, Yélamos J: Human anti-porcine gammadelta T-cell xenoreactivity is inhibited by human FasL expression on porcine endothelial cells. Transplantation. 2001 Aug 15;72(3):503-9.
PubMed
Variant
100 tests
T7-147-T100
In stock
275.00 USD

25 tests
T7-147-T025
Delivery 1 week
137.50 USD
Variant
100 tests
T7-147-T100
In stock
275.00 USD

25 tests
T7-147-T025
Delivery 1 week
137.50 USD

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