Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu TCR Cbeta1 Purified

Anti-Hu TCR Cbeta1 Purified

Anti-Hu TCR Cbeta1 Purified
Regulatory status
RUO
Antigen
TCR Cbeta1
Clone
JOVI.1
Format
Purified
Reactivity
Human
Application
IP, FC (QC tested), IHC(F)
Variant
0.1 mg
11-850-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).
Application
IP, FC (QC tested), IHC(F)
Application details
Flow cytometry: Recommended dilution: 2-6 µg/ml
Reactivity
Human
Immunogen
thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
Other names
TCRCB1, TRBC1
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-850_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).
11-850_FC_Histogram
Separation of human TCR Cbeta1 positive lymphocytes (red-filled) from TCR Cbeta1 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) purified antibody (concentration in sample 1.7 μg/ml, GAM APC).

Product specific references:

Huppa JB, Ploegh HL: In vitro translation and assembly of a complete T cell receptor-CD3 complex. J Exp Med. 1997 Aug 4;186(3):393-403.
PubMed
Viney JL, Prosser HM, Hewitt CR, Lamb JR, Owen MJ: Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice. Hybridoma. 1992 Dec;11(6):701-13.
PubMed
San José E, Alarcón B: Receptor engagement transiently diverts the T cell receptor heterodimer from a constitutive degradation pathway. J Biol Chem. 1999 Nov 19;274(47):33740-6.
PubMed
Pasare C, Mukherjee P, Verhoef A, Bansal P, Mendiratta SK, George A, Lamb JR, Rath S, Bal V: T cells in mice expressing a transgenic human TCR beta chain get positively selected but cannot be activated in the periphery by signaling through TCR. Int Immunol. 2001 Jan;13(1):53-62.
PubMed
Martin EP, Arnaud J, Alibaud L, Gouaillard C, Llobera R, Huchenq-Champagne A, Rubin B: Molecular mechanisms in the TCR (TCR alpha beta-CD3 delta epsilon, gamma epsilon) interaction with zeta 2 homodimers: clues from a 'phenotypic revertant' clone. Int Immunol. 1999 Jul;11(7):1005-15.
PubMed
Variant
0.1 mg
11-850-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Contact distributor
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