Anti-Hu TCR Cbeta1 FITC

Anti-Hu TCR Cbeta1 FITC
Regulatory status
RUO
Antigen
TCR Cbeta1
Clone
JOVI.1
Format
FITC
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
1F-850-T100
Ask for delivery term HERE
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TCRCB1, TRBC1
Antigen description
Alpha-beta T cell receptors (TCRs) are antigen specific receptors, which are essential to the immune response and are present on the cell surface of T lymphocytes. They recognize peptide-loaded major histocompatibility complexes (pMHCs), that are displayed by antigen presenting cells (APCs). Binding of alpha-beta TCR to pMHC initiates TCR-CD3 clustering on the cell surface and intracellular activation of LCK, that phosphorylates the ITAM motifs of CD3gamma, CD3delta, CD3epsilon and CD3zeta, enabling the recruitment of ZAP70. In turn, ZAP70 phosphorylates LAT, which recruits numerous signaling molecules to form the LAT signalosome. The LAT signalosome propagates signal branching to three major signaling pathways, the calcium signaling, the mitogen-activated protein kinase (MAPK) kinase and the nuclear factor NFkappaB (NF-kB) pathways, leading to the mobilization of transcription factors, that are critical for gene expression and essential for T cell growth and differentiation. The T cell repertoire is generated by V-D-J-C rearrangements. This repertoire is then shaped by intrathymic selection events to generate a peripheral T cell pool of self-MHC restricted, non-autoaggressive T cells. Post-thymic interaction of alpha-beta TCRs with the pMHCs shapes TCR structural and functional avidity.
Entrez Gene ID 28639
UniProt ID P01850
1F-850_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).
1F-850_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR Cbeta1 (JOVI.1) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
1F-850_FC_Histogram
Separation of human TCR Cbeta1 positive T cells (red-filled) from TCR Cbeta1 negative lymphocytes(black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) FITC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Huppa JB, Ploegh HL: In vitro translation and assembly of a complete T cell receptor-CD3 complex. J Exp Med. 1997 Aug 4;186(3):393-403.
PubMed
Viney JL, Prosser HM, Hewitt CR, Lamb JR, Owen MJ: Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice. Hybridoma. 1992 Dec;11(6):701-13.
PubMed
San José E, Alarcón B: Receptor engagement transiently diverts the T cell receptor heterodimer from a constitutive degradation pathway. J Biol Chem. 1999 Nov 19;274(47):33740-6.
PubMed
Pasare C, Mukherjee P, Verhoef A, Bansal P, Mendiratta SK, George A, Lamb JR, Rath S, Bal V: T cells in mice expressing a transgenic human TCR beta chain get positively selected but cannot be activated in the periphery by signaling through TCR. Int Immunol. 2001 Jan;13(1):53-62.
PubMed
Martin EP, Arnaud J, Alibaud L, Gouaillard C, Llobera R, Huchenq-Champagne A, Rubin B: Molecular mechanisms in the TCR (TCR alpha beta-CD3 delta epsilon, gamma epsilon) interaction with zeta 2 homodimers: clues from a 'phenotypic revertant' clone. Int Immunol. 1999 Jul;11(7):1005-15.
PubMed
Horna P, Shi M, Olteanu H, Johansson U: Emerging role of T-cell receptor constant β chain-1 (TRBC1) expression in the flow cytometric diagnosis of T-cell malignancies. Int J Mol Sci. 2021 Feb 12;22(4):1817.
PubMed
Variant
100 tests
1F-850-T100
Ask for delivery term HERE
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

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