Anti-Hu TCR Cbeta1 APC

Anti-Hu TCR Cbeta1 APC
Regulatory status
RUO
Antigen
TCR Cbeta1
Clone
JOVI.1
Format
APC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
100 tests
1A-850-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Images
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody JOVI.1 recognizes an extracellular epitope on TCR Cbeta1 (TRBC1).
Application
FC (QC tested)
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
thymus, spleen, and mesenteric lymph nodes isolated from a mouse transgenic for human TCR Vbeta3Cbeta1
Other names
TCRCB1, TRBC1
Preparation
The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1A-850_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) APC antibody (4 μl reagent / 100 μl of peripheral whole blood).
1A-850_FC_Dot-Plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR Cbeta1 (JOVI.1) APC antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3(UCHT1) Pacific Blue™ antibody (4 μl reagent / 100 μl of peripheral whole blood).
1A-850_FC_Histogram
Separation of human TCR Cbeta1 positive lymphocytes (red-filled) from TCR Cbeta1 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Cbeta1 (JOVI.1) APC antibody (4 μl reagent / 100 μl of peripheral whole blood).

Product specific references:

Huppa JB, Ploegh HL: In vitro translation and assembly of a complete T cell receptor-CD3 complex. J Exp Med. 1997 Aug 4;186(3):393-403.
PubMed
Viney JL, Prosser HM, Hewitt CR, Lamb JR, Owen MJ: Generation of monoclonal antibodies against a human T cell receptor beta chain expressed in transgenic mice. Hybridoma. 1992 Dec;11(6):701-13.
PubMed
San José E, Alarcón B: Receptor engagement transiently diverts the T cell receptor heterodimer from a constitutive degradation pathway. J Biol Chem. 1999 Nov 19;274(47):33740-6.
PubMed
Pasare C, Mukherjee P, Verhoef A, Bansal P, Mendiratta SK, George A, Lamb JR, Rath S, Bal V: T cells in mice expressing a transgenic human TCR beta chain get positively selected but cannot be activated in the periphery by signaling through TCR. Int Immunol. 2001 Jan;13(1):53-62.
PubMed
Martin EP, Arnaud J, Alibaud L, Gouaillard C, Llobera R, Huchenq-Champagne A, Rubin B: Molecular mechanisms in the TCR (TCR alpha beta-CD3 delta epsilon, gamma epsilon) interaction with zeta 2 homodimers: clues from a 'phenotypic revertant' clone. Int Immunol. 1999 Jul;11(7):1005-15.
PubMed
Variant
100 tests
1A-850-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD