Anti-Hu TCR alpha/beta PE-Cy™5

Anti-Hu TCR alpha/beta PE-Cy™5
Regulatory status
RUO
Antigen
TCR alpha/beta
Clone
IP26
Format
PE-Cy™5
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
100 tests
T8-607-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody IP26 recognizes a monomorphic extracellular determinant of TCR alpha/beta, the dominant subtype of T cell receptor expressed in human peripheral blood.
Application
Application details
Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Preparation
The purified antibody is conjugated with tandem dye PE-Cy™5 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Cy™ and CyDye™ are registered trademarks of Cytiva.
Other names
TCRA/B
Antigen description
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
T8-607_FC_Profil značení
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR alpha/beta (IP26) PE-Cy™5 antibody (4 μl reagent / 100 μl of peripheral whole blood).
T8-607_FC_Dot-plot
Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR alpha/beta (IP26) PE-Cy™5 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human CD3 (UCHT1) APC antibody (10 μl reagent / 100 μl of peripheral whole blood).
T8-607_FC_Histogram
Separation of human TCR alpha/beta positive lymphocytes (red-filled) from TCR alpha/beta negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR alpha/beta (IP26) PE-Cy™5 antibody (4 μl reagent / 100 μl of peripheral whole blood).

General references:

von Boehmer H: T cell development and selection in the thymus. Bone Marrow Transplant. 1992;9 Suppl 1:46-8.
PubMed
Levelt CN, Wang B, Ehrfeld A, Terhorst C, Eichmann K: Regulation of T cell receptor (TCR)-beta locus allelic exclusion and initiation of TCR-alpha locus rearrangement in immature thymocytes by signaling through the CD3 complex. Eur J Immunol. 1995 May;25(5):1257-61.
PubMed
Michie AM, Zúñiga-Pflücker JC: Regulation of thymocyte differentiation: pre-TCR signals and beta-selection. Semin Immunol. 2002 Oct;14(5):311-23.
PubMed
Croxford AL, Akilli-Ozturk O, Rieux-Laucat F, Förster I, Waisman A, Buch T: MHC-restricted T cell receptor signaling is required for alpha beta TCR replacement of the pre T cell receptor. Eur J Immunol. 2008 Feb;38(2):391-9.
PubMed

Product specific references:

Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009 Jan 8;113(2):358-69.
PubMed
Variant
100 tests
T8-607-T100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD

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