Anti-Hu SUSD2 APC

Anti-Hu SUSD2 APC
Regulatory status
RUO
Antigen
SUSD2
Clone
W5C5
Format
APC
Reactivity
Human
Application
Excitation laser
red (633 nm)
Variant
0.1 mg
1A-835-C100
Ask for delivery term HERE
286.00 USD
Variant
0.1 mg
1A-835-C100
Ask for delivery term HERE
286.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody W5C5 recognizes an extracellular epitope of SUSD2, a type I transmembrane protein expressed on mesenchymal stem-like cells. This antibody selectively binds to a MSCs in both bone marrow and endometrium or tonsil, and can be used for their identification and isolation.
Application
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Human
Immunogen
WERI-RB-1 retinoblastoma cells
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Antigen description
SUSD2 (sushi domain containing protein 2) is a type I transmembrane protein, that serves as an important marker of bone marrow-derived mesenchymal stem-like cells (bone marrow stromal cells). These pluripotent cells are important for techniques of autologous cell therapy, and can be collected from e.g. endometrium, or palatine tonsil. SUSD2 seems to be a tumor supresor, and is down-regulated in colon cancer tissues, whereas it is highly expressed e.g. in breast cancer.
Entrez Gene ID 56241
UniProt ID Q9UGT4
1A-835_FC_Histogram
Separation of HeLa cells stained using anti-human SUSD2 (W5C5) APC antibody (concentration in sample 1.67 μg/ml, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 1.67 μg/ml, same as SUSD2 APC concentration, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Bredenkamp N, Stirparo GG, Nichols J, Smith A, Guo G: The cell-surface marker sushi containing domain 2 facilitates establishment of human naive pluripotent stem cells. Stem Cell Reports. 2019 Jun 11;12(6):1212-1222.
PubMed
Murakami K, Lee YH, Lucas ES, Chan YW, Durairaj RP, Takeda S, Moore JD, Tan BK, Quenby S, Chan JK, Gargett CE, Brosens JJ: Decidualization induces a secretome switch in perivascular niche cells of the human endometrium. Endocrinology. 2014 Nov;155(11):4542-53.
PubMed
Lee BJ, Kang DW, Park HY, Song JS, Kim JM, Jang JY, Lee JC, Wang SG, Jung JS, Shin SC: Isolation and Localization of Mesenchymal Stem Cells in Human Palatine Tonsil by W5C5 (SUSD2). Cell Physiol Biochem. 2016;38(1):83-93.
PubMed
Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun;1106:262-71.
PubMed
Sivasubramaniyan K, Harichandan A, Schumann S, Sobiesiak M, Lengerke C, Maurer A, Kalbacher H, Bühring HJ: Prospective isolation of mesenchymal stem cells from human bone marrow using novel antibodies directed against Sushi domain containing 2. Stem Cells Dev. 2013 Jul 1;22(13):1944-54.
PubMed
Ulrich D, Tan KS, Deane J, Schwab K, Cheong A, Rosamilia A, Gargett CE: Mesenchymal stem/stromal cells in post-menopausal endometrium. Hum Reprod. 2014 Sep;29(9):1895-905.
PubMed
Variant
0.1 mg
1A-835-C100
Ask for delivery term HERE
286.00 USD
Variant
0.1 mg
1A-835-C100
Ask for delivery term HERE
286.00 USD

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