Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu SUSD2

Anti-Hu SUSD2

Regulatory status

RUO

Antigen

SUSD2

Clone

W5C5

Format

Purified

Reactivity

Human

Application

FC (QC tested), ICC

Variant

0.1 mg

11-835-C100

In stock

198.00 USD

Variant

0.1 mg

11-835-C100

In stock

198.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody W5C5 recognizes an extracellular epitope of SUSD2, a type I transmembrane protein expressed on mesenchymal stem-like cells. This antibody selectively binds to a MSCs in both bone marrow and endometrium or tonsil, and can be used for their identification and isolation.

Application

FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Human

Immunogen

WERI-RB-1 retinoblastoma cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Antigen description

SUSD2 (sushi domain containing protein 2) is a type I transmembrane protein, that serves as an important marker of bone marrow-derived mesenchymal stem-like cells (bone marrow stromal cells). These pluripotent cells are important for techniques of autologous cell therapy, and can be collected from e.g. endometrium, or palatine tonsil. SUSD2 seems to be a tumor supresor, and is down-regulated in colon cancer tissues, whereas it is highly expressed e.g. in breast cancer.

Entrez Gene ID 56241

UniProt ID Q9UGT4

Separation of MCF-7 cells stained using anti-human SUSD2 (W5C5) purified antibody (concentration in sample 5,0 μg/ml, GAM APC, red-filled) from MCF-7 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (surface staining).

Product specific references:

Bredenkamp N, Stirparo GG, Nichols J, Smith A, Guo G: The cell-surface marker sushi containing domain 2 facilitates establishment of human naive pluripotent stem cells. Stem Cell Reports. 2019 Jun 11;12(6):1212-1222.
PubMed

Murakami K, Lee YH, Lucas ES, Chan YW, Durairaj RP, Takeda S, Moore JD, Tan BK, Quenby S, Chan JK, Gargett CE, Brosens JJ: Decidualization induces a secretome switch in perivascular niche cells of the human endometrium. Endocrinology. 2014 Nov;155(11):4542-53.
PubMed

Lee BJ, Kang DW, Park HY, Song JS, Kim JM, Jang JY, Lee JC, Wang SG, Jung JS, Shin SC: Isolation and Localization of Mesenchymal Stem Cells in Human Palatine Tonsil by W5C5 (SUSD2). Cell Physiol Biochem. 2016;38(1):83-93.
PubMed

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun;1106:262-71.
PubMed

Sivasubramaniyan K, Harichandan A, Schumann S, Sobiesiak M, Lengerke C, Maurer A, Kalbacher H, Bühring HJ: Prospective isolation of mesenchymal stem cells from human bone marrow using novel antibodies directed against Sushi domain containing 2. Stem Cells Dev. 2013 Jul 1;22(13):1944-54.
PubMed

Ulrich D, Tan KS, Deane J, Schwab K, Cheong A, Rosamilia A, Gargett CE: Mesenchymal stem/stromal cells in post-menopausal endometrium. Hum Reprod. 2014 Sep;29(9):1895-905.
PubMed