Exbio — Research products — Antibodies — CD and related antigens — Anti-Hu STRO-1 Purified

Anti-Hu STRO-1 Purified

Regulatory status

RUO

Antigen

STRO-1

Clone

STRO-1

Format

Purified

Reactivity

Human, Rabbit, Rat

Application

FC (QC tested), ICC

Variant

0.1 mg

11-649-C100

In stock

264.00 USD

0.025 mg

11-649-C025

Delivery 1 week

132.00 USD

Variant

0.1 mg

11-649-C100

In stock

264.00 USD

0.025 mg

11-649-C025

Delivery 1 week

132.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM lambda

Specificity

The mouse monoclonal antibody STRO-1 recognizes an extracellular epitope of the cell surface antigen STRO-1 expressed by bone marrow mesenchymal stromal cells and nucleated erythroid precursors, but not by committed hematopoietic progenitors.

Application

FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml.

Reactivity

Human, Rabbit, Rat

Immunogen

Human CD34 positive bone marrow cells

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

HSC70, HSPA8, LAP1, HSC54, NIP71, HEL-33, HEL-S-72p

Antigen description

STRO-1 is a cell surface antigen expressed by stromal elements in human bone marrow, identified by monoclonal antibody STRO-1. Approximately 10% of mononuclear cells, greater than 95% of which are nucleated erythroid precursors, are STRO-1 positive, whereas the CFU-GM (colony-forming unit granulocyte-macrophage), BFU-E (erythroid burst) and CFU-Mix (mixed colonies) committed progenitor cells are negative. CFU-F (fibroblast colony-forming cells) are present exclusively in the STRO-1 positive population. When plated under long-term bone marrow culture conditions, STRO-1 positive cells generate adherent cell layers containing multiple stromal cell types, including adipocytes, smooth muscle cells, osteoblasts, chondrocytes, and fibroblastic elements. In combination with glycophorin A, STRO-1 is a useful marker for identification of mesenchymal stem cells. STRO-1 and CD117 are markers for osteosarcoma cells.

Entrez Gene ID 3312

UniProt ID P11142

Flow cytometry surface staining pattern of human bone marrow cells stained using anti-human STRO-1 (STRO-1) purified antibody (concentration in sample 4 μg/ml, GAM PE).

Flow cytometry multicolor surface staining pattern of human bone marrow cells using anti-human STRO-1 (STRO-1) purified antibody (concentration in sample 4 μg/ml, GAM PE) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (4 μl reagent per milion cells in 100 μl of cell suspension).

Separation of CD45dim cells stained using anti-human STRO-1 (STRO-1) purified antibody (concentration in sample 4 μg/ml, GAM PE, red-filled) from CD45dim cells unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (surface staining) of human bone marrow cells.

Product specific references:

Oyajobi BO, Lomri A, Hott M, Marie PJ: Isolation and characterization of human clonogenic osteoblast progenitors immunoselected from fetal bone marrow stroma using STRO-1 monoclonal antibody. J Bone Miner Res. 1999 Mar;14(3):351-61.
PubMed

Bensidhoum M, Chapel A, Francois S, Demarquay C, Mazurier C, Fouillard L, Bouchet S, Bertho JM, Gourmelon P, Aigueperse J, Charbord P, Gorin NC, Thierry D, Lopez M: Homing of in vitro expanded Stro-1- or Stro-1+ human mesenchymal stem cells into the NOD/SCID mouse and their role in supporting human CD34 cell engraftment. Blood. 2004 May 1;103(9):3313-9.
PubMed

Kaneko R, Akita H, Shimauchi H, Sasano Y: Immunohistochemical localization of the STRO-1 antigen in developing rat teeth by light microscopy and electron microscopy. J Electron Microsc (Tokyo). 2009 Dec;58(6):363-73.
PubMed

Stenderup K, Justesen J, Eriksen EF, Rattan SI, Kassem M: Number and proliferative capacity of osteogenic stem cells are maintained during aging and in patients with osteoporosis. J Bone Miner Res. 2001 Jun;16(6):1120-9.
PubMed

Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, Kortesidis A, Simmons PJ: Molecular and cellular characterisation of highly purified stromal stem cells derived from human bone marrow. J Cell Sci. 2003 May 1;116(Pt 9):1827-35.
PubMed

Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, Shi S: Recovery of stem cells from cryopreserved periodontal ligament. J Dent Res. 2005 Oct;84(10):907-12.
PubMed

Simmons PJ, Torok-Storb B: Identification of stromal cell precursors in human bone marrow by a novel monoclonal antibody, STRO-1. Blood. 1991 Jul 1;78(1):55-62.
PubMed

Masuda H, Anwar SS, Bühring HJ, Rao JR, Gargett CE: A novel marker of human endometrial mesenchymal stem-like cells. Cell Transplant. 2012;21(10):2201-14.
PubMed